Why are we using this chemicals?(Determination of solubility and soaping number of lipids) Materials: *Fats and oils *Oil solvent (Equal volume 95% ethanol and ether) *0.5 M alcoholic KOH solution *0.05% phenolphthalein solution *0.5 M HCl solution Procedures: Determination of Saponification Number 1) Dissolve 1 g (or 2 mL) of oil in 3 mL of oil solvent and add 25 mL of KOH solution. Put a few boiling stones and boil in the water bath by mixing for 30 minutes. 2) Add 3 drops of phenolphthalein to the saponified and brought to room temperature solution and titrate with HCl. 3)Titrate 25 mL of KOH solution containing 2 mL of water with HCl for a blank test. Solubility of lipids 1) Record the physical properties of all oils before starting the experiment. Then observe the solubility of the oils in water and organic solvents. Discuss the difference. 2) Add 1 mL of water to the oil solutions in ethyl alcohol. Mix vigorously and record your observations in the first second and after 5 minutes.
Why are we using this chemicals?(Determination of solubility and soaping number of lipids)
Materials:
*Fats and oils
*Oil solvent (Equal volume 95% ethanol and ether)
*0.5 M alcoholic KOH solution
*0.05% phenolphthalein solution
*0.5 M HCl solution
Procedures:
Determination of Saponification Number
1) Dissolve 1 g (or 2 mL) of oil in 3 mL of oil solvent and add 25 mL of KOH solution. Put a few boiling stones and boil in the water bath by mixing for 30 minutes.
2) Add 3 drops of phenolphthalein to the saponified and brought to room temperature solution and titrate with HCl.
3)Titrate 25 mL of KOH solution containing 2 mL of water with HCl for a blank test.
Solubility of lipids
1) Record the physical properties of all oils before starting the experiment. Then observe the solubility of the oils in water and organic solvents. Discuss the difference.
2) Add 1 mL of water to the oil solutions in ethyl alcohol. Mix vigorously and record your observations in the first second and after 5 minutes.
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