Q: The rate of migration of nucleic acid fragments through an agarose gel is determined by the_
A: The gel electrophoresis is used to separate DNA fragments based on their size, the gel…
Q: Based on the Agarose Gel Electrophoresis results, tell in which concentration range of KCl the…
A: Ion-exchange chromatography separates molecules based on their charge properties. Based on charge…
Q: Can gelatin hydrolysis be correlated with the pathogenicity of a bacterium? Explain your answer
A: Disclaimer: “Since you have asked multiple questions, we will solve the first question for you. If…
Q: Which of the following tools cannot facilitate the visualization of specific intracellular proteins…
A: Radio isotopes can visualise uptake and downtake but not specific intra cellular proteins. DNA…
Q: Why do cells need to be kept in the freezing devices first then transferred to liquid nitrogen…
A: Rapid freezing of cells can cause ice crystals large crystals can be formed in which cells are…
Q: What is the media used in Catalase test and its expected results?
A: Catalase is an enzyme that is found in the cell in most of the organism that protect the cell from…
Q: A powdered complex medium can be stored for months in the lab. However, after rehydrating the medium…
A:
Q: three biochemical tests that would best differentiate Shigella from E. coli
A: We can perform TSI (Triple sugar iron) test, Lactose fermentation test and motility test to…
Q: X-ray diffraction of enzyme active site explained
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Q: Between Proteus mirabilis and Proteus vulgaris which are positive or negative for ornithine…
A: ornithine decarboxylase test is a special kind of biochemical test that helps to determine whether…
Q: Nitrate reduction test can be used to differentiate between Pseudomonads and Enterobacteriaceae. You…
A: The nitrate reduction test is used to differentiate between Pseudomonads and Enterobacteriaceae or…
Q: What effect does the H2O2 have on bacteia in the catalase test?
A: The production of toxic substances like hydrogen peroxide due to metabolic activities can be lethal…
Q: Differentiate Widal from Weil-Felix test.
A: The Weil-Felix test detects typhus and specific rickettsial infections. Rickettsia is bacteria…
Q: In the amino acid decarboxylase test (lysine decarboxylase), we added mineral oil to the tube before…
A: Introduction The Lysine Decarboxylase Test Is Based On Some Bacteria's Capacity To Convert L-lysine…
Q: If you had cultures of E. coli and E. aerogenes and forgot to label them, which of the following…
A: E. aerogenes is indole-negative but E. coli is indole positive i.e., indole positive bacteria can…
Q: what does it mean when bubbles shows during a catalase test
A: Bacteria are a group of prokaryotic microscopic single celled organisms. They live in diverse…
Q: What other method could be used to purify lysozyme from white using its unusual pl?
A: The unique charge properties of lysozyme with a very high pI of about 10.5 can be used in the…
Q: what type of gel must be used when the protein size is 2500 Da
A: A type of size exclusion chromatography in which the separation of components is based on the…
Q: Lysozyme and benzonase are included in the lysis buffer, given that these are enzymes, one might not…
A: Lysozyme and Benzonase are enzyme and Endonuclease respectively.
Q: For e-coli- Describe how catalase test would have been done (if this test was used)
A: Please note, in accordance with the guidelines, the first question has been answered. Please repost…
Q: Why is it important that the agarose is exposed to ethidium bromide, which is either added to the…
A: Ethidium bromide is utilized during separation of fragments of DNA by agarose gel electrophoresis.…
Q: Each liter of an intranasal antibiotic solution contains 160 mg of gentamicin. You have 2-mL…
A: A number of gram-negative infections are treated with the aminoglycoside antibiotic gentamicin.…
Q: Describe how catalase test would have been done ??
A: Introduction Catalase is the most common enzyme which takes parts in defence against ROS (Reactive…
Q: Oxidase Test: In the document you submit, please describe the results for this test… (like yellow…
A: If organism is oxidase positive then development of purple/ blue color appears in 5-10second. There…
Q: H-Ç-OH HO-C-H HO-5-H H- C-OH HO H ne monosaocharide shown can be re a. tetrose b. tnos
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Q: You're trying to purify a protein with the aid of an ion exchange column. On the column, the protein…
A: The most commonly used technique to separate and purify proteins, nucleic acids, polypeptides and…
Q: does ribose-5-phosphate pass fehlings test?
A: Fehling's test is one of the sensitive test for detection of reducing sugars. Fehling's solution is…
Q: Why does the oxidase test must be performed from blood agar or another medium without a fermentable…
A: Biochemical tests are used to identify microbial diversity based on variations in the biochemical…
Q: Describe how the following technique work on protein purification? ammonium sulfate precipitation
A: Proteins are organic macromolecules that assist in maintaining the structural and functional…
Q: Why differential centrifugation can cause contamination in pellet?
A: Differential centrifugation is a typical process used in microbiology and cytology to separate…
Q: Which test require a straight line inoculation? Choose all that apply. A) Bile esculinase B) NaCl…
A: Inoculation is a form of immunisation that involves the introduction of an infectious material onto…
Q: You inoculate a species of bacteria on a MacConkey agar. It normally shows positive for growth and…
A: MacConkey Agar is a differential agar for Lactose fermentation while if no other Sugars are used,…
Q: In lactose fermentation test what does V means? Please help
A: In the microbiology lab to identify any organism of the coliform group, there will be a test which…
Q: Why Cepacol is used ?
A: Cepacol is a drug which relieves pain associated with a sore throat or mouth. It can be administered…
Q: Suppose a microorganism is growing on a medium that contains amino acids but no sugars. In general…
A: Microorganism requires amino acid in their nutrient for the supply of nitrogen and it helps in the…
Q: What will you observe when the isolated beta amylase enzyme is subjected to biuret test
A: An enzyme is a biocatalyst that increase the rate of chemical reaction without itself being…
Q: What is the result of this biochemical test in the gelatin deep? Which enzyme is this testing for?…
A: Gelatin Hydrolysis Test: Gelatin is a collagen-derived protein obtained from the connective tissues…
Q: After performing the catalase test from question 17, your bacteria produce the result on the RIGHT…
A: Catalase is the tetramer enzyme that breaks the hydrogen peroxide into water and oxygen. This…
Q: If a catalase test turns out positive, what can you say about the cells that were tested?
A: Test is designed to demonstrate the known truth about a situation. It is used for examining the…
Q: Below are the results for your unknown organism:
A: Gram-negative bacteria cause diseases such as pneumonia, blood infections, incision, or wound…
Q: A number of tests are used to identify a bacterial pathogen taken from human patients.Research and…
A: The detection of particular antibodies in combination with the evaluation of clinical symptoms or…
Q: C. Chemical Nature of Polyphenoloxidase Enzyme Activity (Color Intensity) Light pink (Moderate…
A: Biochemical reactions that occur in the living cells are catalyzed by enzymes.
Q: What technique can be used to separate amino acids on the basis of their pI values? (a) Thin-layer…
A: An amino acid is a noramlly an organic chemical. Organic chemicals normally have carbon-hydrogen…
Q: Suppose your Professor handed you a test tube with 2.0mL of E.coli broth culture and told you to…
A: In both basic and applied research, "microbiology" is a highly significant area. Proteomics,…
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- Does Escherichia coli provide positive or negative results for the lysine decarboxylase and ornithine decarboxylase test results?Why is the Mucic Acid test specific for the presence of galactose? Please explain do not copy from google, type the answer if u canUrease breaks down urea into what product? What color is an indication of a positive urease test?