Which of the following tools cannot facilitate the visualization of specific intracellular proteins by light or electron microscopy? a.Radioisotopes b.DNA stains c.Enzyme-substrate tracers d.Flourochromes
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Which of the following tools cannot facilitate the visualization of specific intracellular proteins by light or electron microscopy?
d.Flourochromes
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- Your instructor asks you to isolate and identify the organisms in an unknown culture. You find that the culture contains two gramnegative bacilli that produce swarming colonies. What biochemical test would you use to identify the bacilli? Justify your answer.Which of the following is similar to a block of highly purified “jello-like” substance that is not completely solid and has numerous pores throughout its structure? A. Micropipette b. Restriction enzymes c. Agarose d. Negative electrode please give correct answerIf a student would like to examine if an bacterial organism is capable of using starch, which of the following classes of medium would the student be using? A) A medium classified as differential B) A medium classified as defined C) A medium classified as selective D) A medium classified as minimal E) A medium classified as rich
- Both stained and live organisms are viewed using a microscope, but what is the purpose/benefit of staining a microorganism versus not staining it?If a component is heat sensitive, how might you conveniently achieve sterility of that medium? a. filtration b. batch pasteurization c. autoclave and add the isolated component back afterIn which type of chromatography lipids are carried up a silica gel coated pate by a rising solvent front, less polar travels farther than the more polar ones?a) Absorption chromatographyb) Thin layer chromatographyc) Gas-liquid chromatographyd) HPLC
- In Clinical Chemistry, are enzymatic methods for determining glucose, cholesterol and uric acid more preferred than non-enzymatic methods? Why or why not? In Microbiology, are there technologies that can help make Bacterial Culture and Sensitivity done faster? Provide evidence of use in our country.In sample preparation for electron microscopy, arrange the following steps in correct order A. Apply non-coagulant fixatives to kill cells instantly with minimal structural alterations. B. Wash the specimen with deionized water and then dry it by passing through increasing concentrations of ethanol. C. Expose specimens to uranyl salts to enhance the electron scattering capacity of cellular components. D. Using an ultramicrotome, make ultrathin cuts in the embedded specimen that would be penetrable to electrons. E. Allow the embedding medium to seep into the interstices of the tissue.Please explain the differences between freeze drying,drum drying and air drying and which give a conclusion on which drying method is the best in removal of water in microbial cells.