I need help, with this Bio - Chemistry problem. Please, step by step... Experimental Data:1 ml1 ml1 ml1 ml1 ml1 ml1 ml1 ml1 mlTube 1Distilled waterTube 2BufferTube 3BufferTube 4BufferTube 5KCI0.1Tube 6KCI0.1Tube 7KCI0.2Tube 8KCI0.2Tube 9KCI0.5Agarose Gel:Lane 1MarkerLane 2Tube 2Lane 3Tube 3Lane 4Tube 4Lane 5Tube 5Lane 6Tube 6Lane 7Tube 7Lane 8Tube 8Tube 1 and 9, do not apply. Based on the Agarose Gel Electrophoresisresults, tell in which concentration range ofKCl the restriction enzyme EcoRI eluted in theDEAE Cellulose Anion ExchangeChromatography Step.

Ion-exchange chromatography separates molecules based on their charge properties. Based on charge…

Answered: Based on the Agarose Gel…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Based on the Agarose Gel Electrophoresis results, tell in which concentration range of KCl the restriction enzyme ECORI eluted in the DEAE Cellulose Anion Exchange Chromatography Step.