I did an SDS PAGE with BSA as a standard; I noticed in my results that there is a double band - is there an explanation for this?

Q: technique is used to identify the binding partner for Izumo?

A: Sexual reproduction helps in the increase in progeny of almost all eukaryotic organisms. Sperm meets…

Q: Explain Peptide Mapping and Internal Sequencing of Proteins from Acrylamide Gels.

A: Peptide mapping is an identity test for proteins, those obtained by rDNA technology. It involves the…

Q: Why is R-banding significant when its banding pattern is just the reverse pattern of G-banding?

A: Banding technique: the given banding technique is of chromosome banding techniques i.e. G-banding,…

Q: What would happen if the enzyme rubisco suffers denaturation?

A: Rubisco is the key enzyme  for photosynthetic carbon absorption (assimilation) in catalyzing the CO2…

Q: What are the things we can do to avoid COVID 19?

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Q: In the Lysozyme purification, if the specific activity in the elate was 108 units/mg and the…

A: Egg white is generally rich in lysozyme and hence it is used for its purification. Lysozyme provides…

Q: Heavy metals can denature proteins. What are some denature going agents?

A: Answer Proteins are involved in all almost all biological processes.  For a protein to function…

Q: What does BCR ABL stand for?

A: B-cell receptor (BCR) serve in recognition of the antigens and are present at the surface of the B…

Q: When the gel is removed from the chamber in SDS-PAGE, only the “pre-stained” protein standard is…

A: SDS-PAGE (sodium dodecyl sulphate–polyacrylamide gel electrophoresis), is commonly used as a method…

Q: What would happen during an amino acid sequencing experiment using the Edman degradation if you…

A: The sequence of amino acids can be determined by using amino acids which are present in peptide and…

Q: Can someone give me examples of denatured proteins that can be brought back to native state…

A: The question asks the examples of denatured protein which can be transformed into the renatured or…

Q: Describe in detail the preliminary purification method which you will use to partially purify a very…

A: Proteins are polymers made by combining amino acids as the monomeric units. Amino acids are combined…

Q: With gels, we cannot directly identify the various bands without further analysis. However, we can…

A: Gel electrophoresis can be defined as a process that is used to separate the fragments of DNA based…

Q: This is a picture of an SDS-PAGE gel. In the left lane are protein standards with molecular masses…

A: SDS-PAGE is used determining the molecular weight (MW) of an unknown protein. The first step the…

Q: Which well(A through E) of this agarose gel contains the smallest DNA size? Please look at the pic.

A: Gel electrophoresis technique of separation of molecules on the basis of their size and charged…

Q: How can you fight the covid-19 using this FITT Principle?

A: FITT principle abbreviates as frequency, intensity, time and type.

Q: What are the necessary conditions for the generation okazaki fragments?

A: The short sequences of DNA which are formed by at the lagging strand during replication is called…

Q: In 1925, Briggs and Haidane reported on the modifications of the michaelis-menten equation. Briefly…

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Q: Why were 32P and 35S chosen for use in the Hershey–Chase experiment? Discuss the rationale and…

A: DNA (deoxyribonucleic acid) was discovered by Friedrich Miescher. Nucleotides are the structural…

Q: briefly explain the physical mechanism by which amino acids are separated during paper…

A: Paper chromatography is a type of plane chromatography where the stationary phase is present on a…

Q: Which of the following is defined as transformation?

A: Which of the following is defined as transformation?   Answer. Option (C) . Free-floating DNA is…

Q: why are 2D NMR spectra symmetrical

A: 2D NMR spectra is used to determine the structure of a molecule. It is used for molecules that are…

Q: In urea assay (Catalog #K375-100) what is the nmol (range) it can detect?

A: Urea assay (catalogue #K375-100) is a colorimetric assay used to quantify the urea in any given…

Q: What are two ways to denature a protein and why do these methods work?

A: Given:  Two ways to denature a protein.

Q: What is Initiator element (Inr) ?

A: Nucleic acid is one of the major macromolecules that are present in living organisms. It contains…

Q: 0.175mm e O In the DNA extraction experiment, the separation of DNA from its packaging protein can…

A: Introduction  DNA is crucial biomolecule which serves as genetic material in most of the living…

Q: You are trying to produce a mammalian protein in the bacterium Escherichia coli. To make sure you…

A: A manipulated form of protein is known as a recombinant protein, which is produced in different ways…

Q: What are some advantages of sequencing peptides by mass spectrometry rather than by Edman…

A: Edman degradation:  It is a three-stage reaction that tags and removes the N-terminal residue of a…

Q: Suppose, you have a solution of DNA, RNA and some proteins. How can you separate the proteins from…

A: The protein separation from a mixture is done to study its structural and functional properties. A…

Q: Can native gel electrophoresis of protiens be used to determine protein molecular weight? Explain.

A: Electrophoresis is the motion of dispersed particles relative to a fluid under the influence of a…

Q: What might Watson and Crick have concluded had Chargaff’s datafrom a single source indicated the…

A: DNA is made up of molecules called nucleotides. All the nucleotides contain a phosphate group, a…

Q: What would you expect the functional properties of a riboswitch specific for the amino acid lysine…

A: Riboswitch is a gene sequence present on mRNA, which regulates the protein synthesis by binding to a…

Q: What is the purpose of acrylamide and bis-acrylamide? in SDS-Page Gel

A: Sodium dodecyl sulphate-Polyacrylamide gel electrophoresis (SDS-PAGE) is a common analytical…

Q: What are Okazaki fragments? Why are they formed?

A: DNA replication is the process by which double-stranded DNA is copied to produce two identical…

Q: Draw the potential tautomers of guanine.

A: DNA- DNA acts as the genetic material in most of the organisms . DNA stands for the…

Q: What does it mean to hydrolyze RNA before doing biochemical tests?

A: The nucleic ribose acid is RNA. It is a nucleic acid type. There are three kinds of RNA: reran,…

Q: what are the necessary conditions for the okazaki fragments

A: Nucleic acid synthesis occurs solely within the 5'->3' direction as a result of the…

Q: How liquid chromatography is used in protein purification?

A: Proteins, one of the major categories of macromolecules present in living organisms, are responsible…

Q: Outline the 7 steps of 1H NMR.

A: Nuclear magnetic resonance or NMR spectra tells the number of C and H atoms that are present in a…

Q: After properly troubleshooting, you finally are able to visualize the gel. You obtained the image…

A: INTRODUCTION Gel electrophoresis This is a technique that is used for the separation of DNA…

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A: The major surface glycoproteins used by the influenza virus in order to cause infections are…

Q: why 3 methyl histidine is used to measure muscle mass?

A: 3-methyl histidine is a amino acid derived from the contractile proteins actin and myosin. This  is…

Q: How do you calculate RNA concentration?

A: DNA and RNA are made up of long chains of nucleotides. Sugar molecule, ribose in RNA, and…

Q: Given a mixture of three proteins, A, B and C, with isoelectric points pIA = 4.2, pIB = 7.0 and pIC…

A: The isoelectric point (pI) of a protein is defined as the pH at which the net charge of a protein…

Q: What is an insulator?

A: DNA is the genetic material that carries genetic information in the form of coded nucleotide…

Q: What are Okazaki fragments and how they are formed?

A: As the name suggests, DNA replication is a process that creates two identical copies of the original…

Microbial Isolation

Isolation of the microorganisms in microbiology is the process of developing a pure culture by separating one species of microbe from a mixture of many other species. A pure culture is a culture that contains only one species of microbe.