Given a mixture of three proteins, A, B and C, with isoelectric points pIA = 4.2, pIB = 7.0 and pIC = 7.7, could you separate them using a single chromatographic run?

Principles Of Radiographic Imaging: An Art And A Science
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Chapter8: X-ray Production
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Given a mixture of three proteins, A, B and C, with isoelectric points pI= 4.2, pIB = 7.0 and pI= 7.7, could you separate them using a single chromatographic run?

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