What are the necessary conditions for the generation okazaki fragments?

Q: By what offsets are produced?

A: Plants are non-motile living beings that are capable of producing their own food utilizing the…

Q: What are Ligation points ?

A: Ligation is the joining of two nucleic acid fragments through the action of an enzyme Ligase.

Q: technique is used to identify the binding partner for Izumo?

A: Sexual reproduction helps in the increase in progeny of almost all eukaryotic organisms. Sperm meets…

Q: What is Xanthan ? What is the importance of using Xanthan in the emulsion experiment?

A: Biopolymers are polymers, mainly polysaccharides, which are produced by microbes. They are mainly…

Q: What is the difference between ORF and CDS?

A: ORF (open reading frame) is the portion of a reading frame that has ability to be translated. CDS…

Q: Why is the annealing temperature different between samples? How is this computed?

A: Introduction Polymerase chain reaction or PCR  laboratory technique by which millions to billions of…

Q: Does Escherichia coli provide positive or negative results for the lysine decarboxylase and…

A: Ornithine decarboxylase test detects whether bacteria uses ornithine amino acid for carbon source…

Q: Why is 70% the optimum concentration used in the preparation of ethyl as an antiseptic?

A: Disinfection is referred to as cleaning a surface with a chemical compound so as to destroy…

Q: What is the function of conjugation reactions in the biotransformation process?

A: Biotransformation refers to reactions that modify a specific chemical compound (both endogenous and…

Q: What are the similarity and their differences between Benedict's and Fehling's tests?

A: Benedict's test and Fehling's test are used to determine the presence of sugars in solutions or…

Q: How many microliters of the 500mM colchicine is required for making 1.5mL cell culture with 2mM…

A: Volume of cell culture = 1.5 ml = 1500 microL(V2) (1 ml = 1000 microL) Desired colchicine…

Q: What types of homogenization techniques are available for solubilizing a protein?

A: In the process of slating out, the solubility of the protein reduces due to the highly ionic salt…

Q: Why did Anfinsen use urea and β-mercaptoethanol in hisexperiments?

A: Christian Anfinsen conducted an experiment in the 1950s and determined that specific amino acid…

Q: How would you be able to distinguish between members of the genus Staphylococcus on…

A: Baird Parker agar is used for the isolation and differentiation of staphylococcus species which are…

Q: What is Folate Trap ? Explain the importance of Folate Trap ?

A: In a human body due to deficiency of vitamin B12 folic acid is rendered inactive this phenomenon is…

Q: How did the Hershey–Chase “blender experiments” confirmGriffith’s results?

A: Based on the Griffith experiment's observations, he concluded that genetic material is the…

Q: What is known as montreal protocol?

A: Montreal protocol is an international initiative for ozone depletion.

Q: Outline the steps needed to synthesize the tetrapeptide Ala-Leu-lle-Gly using the Merrifield…

A: Peptide synthesis of significant length needs many steps. Here, the product needs to be purified…

Q: What are revision products? What type of reaction is it? What is the common intermediate reversion…

A: Carbohydrates are complex biomolecules made up of simple building units/monomers called sugars or…

Q: what is the substitution rate? and how is it calculated ?

A: Substitution is type of mutation in which one base pair is been replaced by other base pair.

Q: Each liter of an intranasal antibiotic solution contains 160 mg of gentamicin. You have 2-mL…

A: A number of gram-negative infections are treated with the aminoglycoside antibiotic gentamicin.…

Q: What is nonsense-mediated decay (NMD) ?

A: Given: Non mediated decay is also called as non stop decay. This non stop decay pathway exists in…

Q: How does a dideoxynucleotide differ from a deoxynucleotide? How are dideoxynucleotides important in…

A: Dideoxynucleotides are DNA polymerase chain-elongating inhibitors used in the Sanger technique of…

Q: What types of macromolecules are usually separated on agarose electrophoresis gels?

A: Agarose gel electrophoresis is a method of gel electrophoresis used in biochemistry, molecular…

Q: What is the United States Pharmacopeia requirement for the pH different antacid preparation?

A: Pharmacopeia is a book containing directions for the identification of compound in medicines, and is…

Q: What is the basic principle of GPC (gel permeation chromatography)?

A: Gel permeation chromatography is a type of size-exclusion chromatography that uses organic solvents…

Q: What is the role of imidazole in Ni-Affinity Chromatography?

A: Chromatography is the technique where the mixture of proteins gets separated based on various…

Q: Explain the extraction method and the procedure for extraction of Echinolone by using…

A: Chromatography works on the principle of molecules in a mixture being applied to a surface or a…

Q: What is Shine-Dalgarno sequence? In which groups of microorganisms it is found?

A: Translation is the process of synthesis of protein using mRNA ASA template strand .  Process of…

Q: Under what circumstances should the product Ocuser-pilo be used? Compare the advantages and…

A: The devices that are sterile, thin, multilayered, drug-impregnated and administered into the…

Q: What are the low-molecular-weight precursors needed for the de novo biosynthesis of the purine ring,…

A: Purine nucleotides are synthesized in the body via de novo synthesis or salvage pathway. Adenine and…

Q: What are the benefits and disadvantages of IMAC for purifying proteins?

A: The purification of the proteins is performed by the use of different chromatographic procedures…

Q: What is isosterism and bioisostreism? How does this concept help in biotransformation of different…

A: In biology, drug designing is a major aspect. Drug specificity and effectivity are determined by…

Q: What is the Kozak Sequence?

A: A small amino acid sequence act as an initiation sequence and binds to the mRNA in order to start…

Q: In this exercise, what will be the outcome if B. megaterium was grown in a rich medium? Explain.

A: This is incomplete Question

Q: Why Cepacol is used ?

A: Cepacol is a drug which relieves pain associated with a sore throat or mouth. It can be administered…

Q: What is Kozak sequence ? who invented this ?

A: Introduction  The process of transcribing the mRNA into protein is called Translation. There are 3…

Q: How liquid chromatography is used in protein purification?

A: Proteins, one of the major categories of macromolecules present in living organisms, are responsible…

Q: How does the nucleic acid being purified from associated protein and other impurities?

A: Nucleic acids isolation and purification involve lysis of the cells followed by the removal of…

Q: Which technique is used to identify an unknown protein ?

A: Proteomics is the study of protein analysis. In this study the comparison, of proteins behaviours…

Q: What is reductive dechlorination and why is it environmentallyrelevant?

A: Chlorination refers to the process of the addition of chlorine into the water for the disinfecting…

Q: Why is cobalt chloride paper used in the experiments?

A: The indicators are used in the experiment to detect the distinct observable change. Many types of…

Q: Why is EF-Tu so important in E. coli?

A: Ef- Tu directs the next tRNA to its connects position in ribosomes

Q: What is the expansion of acronym MICA?

A: Immune system includes cells and molecules involved in protecting body against antigens.

Q: What amino acid present in proteins is responsible for the reaction in Hopkins- Cole test ?

A: Amino acids are the monomer of the protein. Each amino acid has a carboxyl group, an amino group, a…

Q: What is annealing?

A: PCR involves three important steps denaturation, annealing and extension. PCR is used for DNA and…

Dissection

Dissection is the process of cutting up the tissue of a dead or live animal or plant, to do anatomical studies and surgical treatments. The purpose of dissection is to learn more about the overall structure of organs and organ systems and thereby understand the physiology of the organism.