I have a 25x stock solution of buffer. How many ml do I add to water to make 50ml of 1x buffer O A. 1 ml 25x stock, 49 ml water B. 10 ml 25x stock, 40 ml water C. 2 ml 25x stock, 48 ml water D. 25 ml 25x stock, 25 ml water O E. 5 ml 25x stock, 45 ml water
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- When acids are added to a solution, the pH should ___________ . a. decrease b. increase c. stay the same d. cannot tell without testingWhen dissolved in water, an _________ donates H+; an ________ accepts H+. a. acid; base c. buffer; solute b. base; acid d. base; bufferA buffer system .... a) All options are correct b) Counteracts pH changes in a solution c) Does the blood protein work as d) Releases protons (H +) if pH increases
- You have a 6 M solution of stock A. You do the following serial dilutions: Add 1 ml of stock A to tube B. Add 9 ml H2O. Mix.Then take 1 ml from tube B and put it into tube C. Add 19 ml H2O. Mix. Then put 5 ml from tube C into tube D. Add 5 ml H2O. A. what is the final (cumulative) dilution of stock A (dilution factor)? B. What is the final concentration (include units) after all these dilutions?Which of the following is the best explanation for a gel like this?A. Bromophenol blue was not added in the loading buffer.B. Glycine was not present in the buffer system.C. There was too much bisacrylamide in the gel solution.D. Gel was not overlaid after pouring the first gel solution.Is there a difference between pH measurements using the pH meter and the pH paper? Compare the initial pH and the final pH of each sample. What was the most effective buffer? What was the least effective? Solución pH inicial pH final Diferencia water 7.45 2.48 0.1 M NaCl 7.28 2.03 milk 6.78 6.45 buffer de fosfato 8.76 7.78 **** Calculate the difference (subtract) the initial pH - final pH values to identify the effectiveness of each solution as a buffer. Values with little difference mean that a less drastic change occurred and are therefore more effective. On the contrary, values by much difference reflect drastic changes in pH and are less effective. What do buffers do and why are they important in biological systems?
- A stock solution was diluted in two steps. In a first step, 0.5 ml was diluted with 9.6 mL buffer. In a second step, 0.5 mL from the first step was diluted with 9.6 mL of buffer. The concentration of the mixture of the second step was determined to be 0.24 mg/mL. What was the concentration of the stock solution?1. Tools that do not need to be brought when sampling is…a. thermometerb. Hygrometerc. PH meterd. DO meter 2. In the deposition method, one of the conditions that must be met is…a. The precipitate has a high solubility or solubility productb. The precipitate formed must be quite difficult to dissolvec. The precipitate formed does not have to be quite pured. Free precipitate formA pH of 3 is a. basic b. neutral c. acidicd. a buffer
- I am unsure how to answer question number 2: What volume of 0.100 M HCl would have to be added to this buffering system, to reduce the pH to 7.1?Using my chart can you help me answer this question? Why did we select distilled water to be the negative control?When loading samples onto a gel, why should expelling all the liquid in the tip be avoided? a)All the sample could be forced out from the well in which it was loaded. b)The extra liquid would end up in the buffer and this would distort the migration of the sample. c)The gel could tear and distort the migration of the sample. d)An air bubble could result which would distort the migration of the sample .