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I need to make 1 liter of the solution.  I need a list of what I need to use and how much of each. It's the AH solution

I need to make 1 liter of the solution.  I need a list of what I need to use and how much of each. It's the AH solution

Principles of Instrumental Analysis
Principles of Instrumental Analysis
7th Edition
ISBN:9781305577213
Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Chapter28: High-performance Liquid Chromatography
Section: Chapter Questions
Problem 28.15QAP
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I need to make 1 liter of the solution.  I need a list of what I need to use and how much of each. It's the AH solution 

Previous Page 11:14 Thu, Jun 23 M ₁, UP Sylgard | Surgica Prev PMC Alt PDF 1:50 / 10:15 Protocol с Download video file. (122M, mp4) Before you start Page 2 of 10 Visualizing the BOX Next artificial hemolyr X ||| artificial hemoly ncbi.nlm.nih.gov/pmc/articles/PMC3150055/#!po Visualizing the Beating Heart in Drosophila J Vis Exp. 2009; (31): 1425. jove Adult hearts 1. Freshly prepare artificial hemolymph (AH) solution containing 108mM Na+, 5mM K+, 2mM Ca2+, 8mM MgCl2, 1mM NaH₂PO4, 4mM NaHCO3, 10mM sucrose, 5mM trehalose, 5mM HEPES (pH 7.1, all reagents from Sigma Chemicals). The sucrose and trehalose should be added to the AH from refrigerated stock solutions just prior to use in order to prevent bacterial contamination. 2. Bring AH to room temperature and oxygenate the solution by air- bubbling for at least 15 min. [] < ↓ 38% 유 + : Next Z Page
Transcribed Image Text:Previous Page 11:14 Thu, Jun 23 M ₁, UP Sylgard | Surgica Prev PMC Alt PDF 1:50 / 10:15 Protocol с Download video file. (122M, mp4) Before you start Page 2 of 10 Visualizing the BOX Next artificial hemolyr X ||| artificial hemoly ncbi.nlm.nih.gov/pmc/articles/PMC3150055/#!po Visualizing the Beating Heart in Drosophila J Vis Exp. 2009; (31): 1425. jove Adult hearts 1. Freshly prepare artificial hemolymph (AH) solution containing 108mM Na+, 5mM K+, 2mM Ca2+, 8mM MgCl2, 1mM NaH₂PO4, 4mM NaHCO3, 10mM sucrose, 5mM trehalose, 5mM HEPES (pH 7.1, all reagents from Sigma Chemicals). The sucrose and trehalose should be added to the AH from refrigerated stock solutions just prior to use in order to prevent bacterial contamination. 2. Bring AH to room temperature and oxygenate the solution by air- bubbling for at least 15 min. [] < ↓ 38% 유 + : Next Z Page
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