Question
Asked Oct 31, 2019

Identify reactions within glycolysis and Citric Acid cycle that produce ATP via substrate level phosphorylation 

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Expert Answer

Step 1

Glycolysis is the process of reaction by which one molecule of glucose is converted to two molecules of pyruvate in which a sequence of 10 enzyme-catalyzed reactions occurs. In this process the net production of 2 ATP and the reduction of 2 NAD+ to 2 NADH occur. The citric acid cycle is a metabolic pathway of oxidizing glucose molecule to yield energy. Glucose is converted to pyruvate in the glycolysis pathway which enters the citric acid cycle as acetyl CoA for its complete oxidation. The citric acid cycle is also called tricarboxylic acid (TCA) cycle or Krebs cycle. Substrate-level phosphorylation refers to the formation of ATP from ADP and a phosphorylated intermediate, rather than from ADP and inorganic phosphate, Pi, as is done in oxidative phosphorylation.

Step 2

During glycolysis, the first substrate-level phosphorylation occurs after the conversion of 3-phosphoglyceraldehyde and Pi and NAD+ to 1,3-bisphosphoglycerate in presence of enzyme  glyceraldehyde 3-phosphate dehydrogenase. 1,3-bisphosphoglycerate is then dephosphorylated via phosphoglycerate kinase, producing 3-phosphoglycerate and ATP through a substrate-level phosphorylation. This is the 6th step of ...

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