Identity gene 1 (bl, pr, vg) Identity gene 2 ( bl, pr, vg) Identity gene 3 ( bl,pr, vg) How many map units separate genes 1 and 2 ( Distance 1)? How many map units separate genes 2 and 3 ( distances 2) ?

Human Heredity: Principles and Issues (MindTap Course List)
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Chapter6: Cytogenetics: Karyotypes And Chromosome Aberrations
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Identity gene 1 (bl, pr, vg) Identity gene 2 ( bl, pr, vg) Identity gene 3 ( bl,pr, vg) How many map units separate genes 1 and 2 ( Distance 1)? How many map units separate genes 2 and 3 ( distances 2) ?
Linkage Mapping Using a Trihybrid Testcross in Fruit Flies
Remember the black, purple, and vestigial genes from Exercise 1? Now you're going to have to construct a map of the same region from
trihybrid testcross data. The new map should be similar to the one from Exercise 1, but it won't be identical because we're starting with a
new, independent data set.
BLACK PURPLE VESTIGIAL TRIHYBRID TESTCROSS
In the parental generation, you mate a pure-breeding wild-type female (bl+/bl*pu*/pu+vg+/vg+ with a pure-breeding black, purple,
vestigial male (bl/bl;pu/pu vg/vg) to produce an F1 generation that is all wild-type (bl/bliput/pu;vg+/vg). Note that the F1 flies are all
trihybrid.
Next, you mate several F1 trihybrid females (bl/bl:put/pu;vg+/vg) with tester males, which are black, purple, vestigial (bl/bl;pu/pu;vg/vg).
The offspring of this trihybrid testcross are:
Phenotype
Wild-type
Black, purple, vestigial
Vestigial
Black, purple
Purple, vestigial
Black
Purple
Black, vestigial
bl pu vg
bl pu vg
Genotype Tester Gamete Trihybrid Gamete Number
+/bl;+/pu;+/vg
bl pu vg
bl/bl;pu/pu;vg/vg
+/bl;+/pu;vg/vg
bl/bl;pu/pu;+/vg
+/bl;pu/pu;vg/vg
bl/bl;+/pu;+/vg
+/bl; pu/pu;+/vg
bl/bl;+/pu;vg/vg
bl pu vg
bl pu vg
bl pu vg
bl pu vg
bl pu vg
+++
bl pu vg
+ + vg
bl pu+
+ pu vg
bl ++
+ pu +
bl + vg
2332
2333
338
337
151
171
24
29
A
the questions in the worksheet that you downloaded in
Transcribed Image Text:Linkage Mapping Using a Trihybrid Testcross in Fruit Flies Remember the black, purple, and vestigial genes from Exercise 1? Now you're going to have to construct a map of the same region from trihybrid testcross data. The new map should be similar to the one from Exercise 1, but it won't be identical because we're starting with a new, independent data set. BLACK PURPLE VESTIGIAL TRIHYBRID TESTCROSS In the parental generation, you mate a pure-breeding wild-type female (bl+/bl*pu*/pu+vg+/vg+ with a pure-breeding black, purple, vestigial male (bl/bl;pu/pu vg/vg) to produce an F1 generation that is all wild-type (bl/bliput/pu;vg+/vg). Note that the F1 flies are all trihybrid. Next, you mate several F1 trihybrid females (bl/bl:put/pu;vg+/vg) with tester males, which are black, purple, vestigial (bl/bl;pu/pu;vg/vg). The offspring of this trihybrid testcross are: Phenotype Wild-type Black, purple, vestigial Vestigial Black, purple Purple, vestigial Black Purple Black, vestigial bl pu vg bl pu vg Genotype Tester Gamete Trihybrid Gamete Number +/bl;+/pu;+/vg bl pu vg bl/bl;pu/pu;vg/vg +/bl;+/pu;vg/vg bl/bl;pu/pu;+/vg +/bl;pu/pu;vg/vg bl/bl;+/pu;+/vg +/bl; pu/pu;+/vg bl/bl;+/pu;vg/vg bl pu vg bl pu vg bl pu vg bl pu vg bl pu vg +++ bl pu vg + + vg bl pu+ + pu vg bl ++ + pu + bl + vg 2332 2333 338 337 151 171 24 29 A the questions in the worksheet that you downloaded in
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