Which of the following takes the LONGEST to kill a live culture of vegetative bacterial cells? a) 170°C in a dry oven b) 121°C in a steaming chamber c) 100°C in a water bath d) Direct exposure to gas flame
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- The bacterium that causes Hansen’s disease (leprosy), Mycobacterium leprae, infects mostly the extremities of the body: hand, feet, and nose. Would this bacteria’s optimum temperature of growth be above or below the core temperature of the human body?During the fall 2001 bioterrorist attack in which anthrax endospores were sent through the mail, one news commentator suggested that people should iron all their incoming mail with a regular household iron as a means of destroying endospores. Would you agree that this is a good way to disinfect mail? Explain your answer. Which disinfectant methods would be both more effective and more practical?placing bacteria at ultracold temperatures is as effective at killing microbes as the use of dry heat in incineration. is is true/false?
- When the microbes were exposed to different temperatures how was it possible to see minimal growth? What had to be functional in order for growth to occur?Among these five areas in the laboratory: air, faucet, table, floor, table, the doorknob, which is expected to have the most number of microorganisms? and which among the areas may contain potentially pathogenic microbes? Provide an explanation.A microbiologist used the concept of enrichment culture to isolate aerobic and anaerobic nitrogen-fixing bacteria, sulfate-reducing bacteria, and sulfur-oxidizing bacteria. What kind of selective media could he haveused for isolating each of these four classes of microbes?
- Which of the following bacteria can survive in in temperatures ranging from 390F (40C)---to 990F (37OC)?In an experiment, the bacteria were placed in dropper bottles containing glycerol as a carbonsource. Why did the culture have to be incubated for an hour in the new media?Some potentially pathogenic bacteria and fungi, including strains of Enterococcus, Staphylococcus, Candida, and Aspergillus, can survive for one to three months on a variety of materials found in hospitals, including scrub suits, lab coats, plastic aprons, and computer keyboards. What can hospital personnel do to reduce the spread of these pathogens?
- What is the best growth medium that contains glucose that can be used to acclimatize bacteria? and why?Of what practical importance are air borne microorganisms to the laboratory workers? What precautions should be taken to control laboratory contaminants? Why are petri dishes incubated in an inverted position? Of what advantage is the using a solid and a liquid medium?Of what importance are spore-forming bacteria in the food industry?