Consider the growth curve shown below. Cells were grown in a medium containing 1 % w/v glucose and 0.5% w/v acetate. Calculate the generation time when growing on glucose and also when growing on acetate. Pay particular attention to the split time axis with different time scales. Glucose generation time = hrs Acetate generation time = hrs 1011, 1010- 109 bacteria
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- Differentiate the growth curve exhibited by the same inoculum under the following conditions. Draw and label the parts of your growth curve. A). If it is exponentially growing and inoculated in the same nutritious medium under the same optimum conditions. B). If it is taken from an old culture and inoculated into the same nutritious medium and incubated under the same optimum conditions in (A). C). If it is taken from an old culture and inoculated to a different and less nutritious medium and incubated under the same optimum conditions as (A).Examine the graph shown below of a liquid culture growth curve for S. pneumoniaegrowing in minimal broth containing both glucose and lactose in equal concentrations as a potential carbon source. Answer the following questions: a) Name the growth phases labelled as I, II and III, explain the observed pattern of growth at each of these stages in the curve b) Where would the concentration of cAMP be low and why? c) In what phase would β-galactosidase be high and why? d) What kind of overall growth pattern does this curve illustrate?Long explanations are not needed. Direct answers would suffice. a. Flux through the pentose phosphate pathway is controlled mainly by the NAD*/NADPH ratio in the cell. I. True II. False b. Flux through the pentose phosphate pathway is controlled mainly by the NAD*/NADPH ratio in the cell. I. True II. False
- Compare and contrast the glucose oxidase and hexokinase methods of glucose determination and relate these procedures to the clinitest method. Please Type Thanks Don't copy any whereTwo microorganisms that use naphthalene as a food source have the same Umax but different Ks values for naphthalene. If both types of microorganisms are inoculated at the same cell density into a liquid culture containing naphthalene as the sole carbon source (i.e. the only food source), which microorganism will outcompete the other: the one with the higher Ks or the one with the lower Ks. Why?.Draw the batch bioreactor used in the production of Parmesan cheese in a simple form.
- A bacterial culture is grown using either octadecane (C18H38) or pentachlorophenol (C6HOCl5)) as the sole source of carbon and energy. The cell yield value is determined by dry weight analysis to be 1.49 for octadecane and 0.05 for pentachlorophenol. Using either octadecane or pentachlorophenol, please describe the steps taken (and the final result) showing what percentage of the substrate carbon will be found as cell mass and as CO2? Please show all calculations.A bacterial culture is grown using either octadecane (C18H38) or pentachlorophenol (C6HOCl5)) as the sole source of carbon and energy. The cell yield value is determined by dry weight analysis to be 1.49 for octadecane and 0.05 for pentachlorophenol. Using either octadecane or pentachlorophenol, please describe the steps taken (and the final result) showing what percentage of the substrate carbon will be found as cell mass and as CO2? Please show all calculations and give correct answers.A bacterial culture is grown using either octadecane (C18H38) or pentachlorophenol (C6HOCl5)) as the sole source of carbon and energy. The cell yield value is determined by dry weight analysis to be 1.49 for octadecane and 0.05 for pentachlorophenol. Using either octadecane or pentachlorophenol, please describe the steps taken (and the final result) showing what percentage of the substrate carbon will be found as cell mass and as CO2? Please show all calculations and give correct answers. Please answer the question.
- The daily production of a growth-associated byproduct (P) is 250 kg/day. The continuous bioreactor without recycle is fed with a substrate solution (S) with a concentration of 60 g/L at a flow rate of 500 L/h. In steady state condition, calculate: a) the product concentration (Cp).b) the conversion of the limiting substrate.c) the cell concentration (Cx).d) the volume of the bioreactor.Data μmax = 0.20 h-1, KS = 0.70 g.L-1, YX/S =0.50 gX/gS, YP/S = 0.35 gP/gS(answers: Considering P0 = 0 and X0 = 0: a) P = 20.83 g/L; b) 99.2% conversion; c) X = 29.76 g/L; d) V = 6071 L)1. Describe the role of sodium and phosphomolybdate reagent in Folin Wu method. 2. Describe the accuracy and specificity of Nelson-Somogyi method 3. Do you think PFF should be part of enzymatic method of glucose determination? Explain your answer.Calculate the Vmax of the above enzyme (lactase). (the enzyme lactase has a Vo of 0.111111111111 mM per minute when [S] = 1.0 mM, and a Vo of 0.20 mM per minute when [S] = 5.0 mM) 0.125 mM per minute 0.25 mM per minute 0.50 mM per minute 1.25 mM per minute 5.0 mM per minute