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- Calculate the biochemical standard cell potential for the oxidation of NADH by molecular oxygen O2 + 2NADH + 2 H+ → 2H2O + 2NAD+Biochemists consider the citric acid cycle to be the cen-tral reaction sequence in metabolism. One of the key steps isan oxidation catalyzed by the enzyme isocitrate dehydrogenaseand the oxidizing agent NAD. Under certain conditions, thereaction in yeast obeys 11th-order kinetics Rate=k[enzyme][isocitrate]⁴[AMP]²[NAD⁺]m[Mg²⁺]² What is the order with respect to NAD⁺?Pls help ASAP, thanks! "Yeast yields ethanol from pyruvate in two steps. Step one requires the co-enzyme __ , which is necessary for the substrate to undergo oxidative decarboxylation."
- BioKemi!! Make a theoretical calculation of how many ATP molecule of acetyl-CoA to Carbon dioxide and water if the entire proton gradient across the mitochondrias inner membrane can be used for ATP production and when the ATP synthase has 15 c-subunits? All steps in the calculations must be reported Hand written solution otherwise down voteWrite balanced biochemical equations for all the reactions in the catabolism of glucose to two molecules of pyruvate (the preparatory & payoff phase of glycolysis), including the standard free-energy change ΔG for each reaction. Then write the overall or net equation of glycolysis, with the net standard free-energy change when one molecule of glucose is converted into 2 molecules of pyruvate.Determine the total yield of ATP for the complete oxidation of 1 molecule of 3-phosphoglycerate. Show the work and reasoning, and indicate how many ATP, NADH, and FADH2 are formed and in which steps. Include ATP produced in the electron transport chain. Express the answer as a range
- Calculate the total energetics involved in the complete beta-oxidation of one mole of 19C fatty acid. Indicate the number of cycles and the number of acetylCoA produced. Indicate also the number of ATPs used. Indicate clearly these reactions which are important for the generation of ATP. Tabulate neatly these reactions.Chemistry Most of the reactions in gluconeogenesis are the simple reversal of the ‘forward’ reactions used in glycolysis. However, formation of PEP in gluconeogenesis from pyruvate by pyruvate kinase is not feasible because the DG of this reaction is too unfavourable. Describe the source(s) of the energy used in gluconeogenesis to drive the production of PEP.Draw the mechanism for the complete oxidation of a C-7 saturated fatty acid. Indicate the yield of ATP equivalents assuming there are 8 c-subunits in the ATP synthase, and NADPH = NADH.
- a) Calculate the enzyme and specific activity of a reaction with 3 μM Hsp90 using the following information: The rate is measured in a spectrophotometer as 0.028 OD units/min in a 1 ml reaction volume. The absorbance was detected at 340nm and the extinction coefficient for NADH at this wavelength is 6200 L M-1 min-1 and the molecular mass of Hsp90 is 82.7 kDa. The rate of NADH utilisation is equivalent to the rate of ATP utilised by Hsp90. Show all your calculations and the units for your answers. b) Calculate the turnover number for the reaction described in (a) aboveAlthough phosphoesters are generally not energetic enough to donate the phosphate to make a high energy bond (e.g., a phosphoanhydride), the phosphate in PEP is used to convert ADP to ATP in the pyruvate kinase reaction. Carefully explain the ‘quirk’ of this reaction that makes it energetically feasible.Saccharides: Using the following substrates, estimate the net ATP yield after glycolytic pathway, Kreb’s cycle and electron transport chain. Assume that the estimate for ATP yield per mole of NADH is 3 moles of ATP, while 1 mole of FADH2 is equivalent to 2 moles of ATP, and one mole of GTP is equivalent to one mole of ATP. Show all pertinent solutions and determine: a) ATP used, b) ATP produced, and c) Net ATP. Based on your solutions, rank the substrates based on increasing yield of ATP 1. Three moles of glucose-6-phosphate