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If you placed one copy of your gene into a tube and ran a PCR for 3 cycles, how many copies of your gene would you have at the end?
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- Assuming you started with only one copy of a DNA sequence, how many copies should be present after 15 cycles of PCR?If you performed PCR to amplify a gene region, and you began with 20 copies of double stranded genome, after 18 cycles, about how many double stranded copies of that region would you have (in theory)?At the end of an experiment, you extract DNA from ten yeast colonies. You divide the DNA from each colony into two tubes, and send all twenty samples for sequencing. How many experimental replicates do you have?
- How many DNA molecules would exist after 20 PCR cycles?If a PCR is started using 10 pieces of template DNA, how many pieces of DNA would there be after 10 cycles? a. About 100 b. About 1000 c. About 10,000 d. About 1010You would like to amplify the gene shown below using PCR. What is the sequence of the primer you would generate for the left side of the sequence? a) 5’ GCGGTTAA 3’ b) 5’ CGCCAATT 3’ c) 5’ TTAACCGC 3’ d) 5’ AATTGGCG 3’
- How did the private corporation Celera Genomics approach the sequencing of the human genome? What was the advantage of this approach?In pcr experiment, Does electrophoresis show that only DNA products of the desired size are present? If not, what do you think is the reason?If you had the ability to do gene editing with ONE gene for the betterment of human kind, which one would you choose, and why? Assume you could either change an abnormal allele associated with a disease, such as the cystin gene associated with Cystic Fibrosis to its normal wild type, or add a pre-existing human allele to a genome.
- In PCR, the goal is to make copies of?What is Recombinant DNA technology ? Who created this ?During DNA isolation, you incubated the tissue sample at 55°C for 60 minutes, followed by incubation at 95°C for 10 minutes. What happens to the DNA molecules in this last 95°C incubation? What would happen to the PCR if this step was omitted?