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- Briefly explain the rationale for the addition of 2N sulfuric acid in the assay of ascorbic acid.Draw the structure of the predominant form (principal species) of 1,3-dihydroxybenzene at pH 9.00 and at pH 11.00. What is the second most prominent species at each pH?What would happen to the your Kc values if you used an absorptivity constant of 6000 M-1, when the actual value was 5000 M-1? Explain your answer?
- 2c)Outline a method based on extraction techniques for the separation of a mixture of compound B from problem I., and compound A from problem II.Why is there a need to add concentrated H2SO4 to the centrifugate and evaporating almost to dryness before the addition of methyl alcohol in confirming the presence of BO3-3?what is the general charge on a Sialic acid at pH=7? please provide calculation and structure.
- 5) Indicate the type of column (packing) to be used to separate the following mixture of carboxylic acids by HPLC: benzoic, thallic, 2,4,6-trichlorobenzoic, ortho and para nitrobenzoic acids.At normal temperature, 5-bromoacetylsalicylic acid melts at 60 °C and is inert and nearly insoluble in water. Why is it that water cannot be utilized as a recrystallizing solvent for this compound?For an acid-base experiment with access to DCM, HCl & NaOH,trying to seperate a mixture of caffeine, benzoic acid and cellulose, can someone explain how this experiment can be conducted? with the chemicals included andinstrumentation
- The melting point of Borneo is 206-209oC and 212-214oC for isoborneol. What could we predict for the melting point range of the product isolated from our experiment, the reduction of camphor with NaBH4?5-bromoacetylsalicylic acid melts at 60 °C and is inert and almost insolube in water at room temperature. Why can’t water be used as recrystallizing solvent for this compound?The order of addition of the bases is important. What would have happened if you instead treated the initial mixture of benzoic acid, 2 - napthol and 1,4-dimethoxybenzene with a 1M NaOH aqueous solution instead of the saturated NaHCO3 solution? How would the separation of the compounds be affected? What would change?