III. True or False a. Large proteins travel/migrate faster in both gel filtration chromatography and SDS-PAGE. b. In cation exchange chromatography, positively charged chemical groups are covalently bound to the polymer beads.
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- Gel-filtration chromatography is a useful method for removing salts, such as ammonium sulfate, from protein solutions. Describe how such a separation is accomplished.What is the basis for the separation of proteins by the following techniques? (a) gel-filtration chromatography (b) affinity chromatography (c) ion-exchange chromatography (d) reverse phase HPLCBriefly explain the principle behind the following techniques:(a) ion-exchange chromatography (b) gel filtration chromatography (c) affinity chromatography
- 1. Calamine Lotion is classified as a suspension. What is a suspension? A. It is a disperse system in which solid, vehicle-insoluble particles (internal phase) are uniformlysuspended by mechanical agitation and formulation design throughout the liquid vehicle(external phase).B. It is a clear, flavored, sweetened, hydroalcoholic liquid oral preparation that usually containeither potent or unpleasant tasting drugsC. It is a concentrated aqueous solution containing one or more sugar components, chiefly sucrose,or sugar substituteD. It is a viscous oral liquid containing one or more medicament dissolved in a vehicle that usuallycontains high proportion of sucrose or other sugars.Explain how gel filtration chromatography works. What type of gel will you used when the protein size is 2500 Da? Explain.Describe step by step process of: a. Ion-exchange Chromatography b. Hydrophobic Interaction Chromatography c. Affinity Chromatography d. Reversed-Phase Chromatography e. Principle of Polyacrylamide Gel Electrophoresis f. Isoelectric Focusing g. Protein Sequencing
- What is the basis for the separation of proteins by the following techniques? (a) gel-filtration chromatography(b) affinity chromatography(c) ion-exchange chromatographyWith gels, we cannot directly identify the various bands without further analysis. However, we can make some assumptions off of their sizes. In order to determine their sizes, we need to add -?- to the gels. a ethidium bromide b loading dye c molecular markers d Coomassie blueDrug X is available in two forms with the following aqueous solubilities: slightly solutble WA or a soluble Na salt of the WA. Which form of the drug is most appropriate for an oil based solution dosage form? a. WA b. Na salt of WA
- There can be more than one correct answer Which of the following methods can seperate proteins based on their mass? a.) centrifugation b.) gel filtration chromatography c.) SDS polyacrylamide gel electrophoresis d.) ion exhange chromatography1. Prepare 200 mL of an isotonic aqueous solution of thimerosal (MW 404.84; L iso 3.4). The concentration of this antiinfective drug is 1:5000. a. Calculate the E value. b. Adjust the Isotonicity of the solution using NaCl.Given this, if you used 6g of vitamin Z powder to make 20 ml of solution, what is the % concentration of this solution? (I gave the image since I don't know if that info is needed to solve this question.)It also gives a follow-up, if you can help here too: You work in a lab as a summer student. One of your tasks is to make sure that there is enough cell culture medium containing antibiotics to grow bacteria. One day you realize that there is only 5 ml of 10% Antibiotic stock solution in the freezer. You decide to use it all to prepare the working culture medium with 0.01% antibiotic. In the lab there is plenty of growth medium without antibiotics. (Note: dilution in medium is like dilution in water). You remember the equation to make dilutions of stock solutions. You usually use this formula to calculate the required volume of a stock solution, but you realize it can apply here as well, even though the unknown is the final volume. So, you make that dilution. Given that each bacterial…