A HEPTAPEPTIDE that punctures the bacterial cell wall has just been recentlyisolated from the venom gland of a frog. Amino acid analysis of the peptide revealsthe following information below: (NOTE: when the sequence is not known, a commaseparates the amino acids)HydruzineAcid Hydrelysis (6 N NC)ot 110°CHeptapeptide(2) Arg. Pro, Phe, Tyr, Ala, Lys2,4-dinitrefluorobenzeneDNP-PheLys andmodified freePeptide 1(Ala, Lys)amino acidsCleavage with Trypsin, then chrometegraphyPeptide 2(Pro, Arg, Tyr)Peptide 3(Phe, Arg)Phe(leavege with (hymotrypsin, then chromotogrophyPeptide 4(Arg, Tyr)Peptide 5(Lys, Pro, Ala, Arg) When answering the questions below, please use the ONE-LETTER CODE for the aminoacid, with NO spaces and symbols between each letter.1. What is the C-terminal residue?2. What is the N-terminal residue?3. What is the sequence of Peptide 2?4. What is the sequence of Peptide 4?5. What is the overall amino acid sequence?

Proteins are composed of amino acids, which are bound together by peptide linkage. Amino acids…

Answered: A HEPTAPEPTIDE that punctures the…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Examine the following peptide and the inserted table, and answer the 2 questions below: Thr-Glu-Pro-Ile-Val-Ala-Pro-Met-Glu-Tyr-Gly-Lys 1. Estimate the net charge of the peptide at pH 2.0. Explain. 2. Estimate the net charge of the peptide at pH 7.0. Explain.
Draw the structural formulas of the following peptides, andshow which bond would be cleaved by chymotrypsin.a. trp-val-glyb. phe-ala-pro
What are the charges of the following amino acids peptides at ph 14? 1. GLAVV 2. RRKKQ
Residues such as valine, leucine, isoleucine, methionine, andphenylalanine are often found in the interior of proteins,whereas arginine, lysine, aspartic acid, and glutamic acid areoften found on the surface of proteins. Suggest a reason forthis observation. Where would you expect to find glutamine,glycine, and alanine?
The following peptide that has been acetylated at the C-terminus: CH3C(O)-GEEGH-NH2 Plot the titration curve for pH as a function of mols NaOH added, indicating the relevant pKa values and the pI value on the graph, label all axes appropriately.
Consider a short peptide with the sequence M-C-Q-L-Y-P-E-D-K. List the ionizable groups in this peptide, and the net charge of the whole peptide at pH a) 1.5, b) 7.0, and c)12.0.
Calculate the net charge of the following peptide at pH 2, pH 6 and pH 14 (use 2 and 9 as pKa values for the carboxylic acid and amino groups, respectively, use the attached table for the side chain pKa values). Ile-Leu-Ile-Lys-Glu-Cys-His-Glu-Met-Ile-Ser-Thr-Arg-Tyr
Determine the amino acid sequence of a polypeptide from the following data:Complete hydrolysis of the peptide yields Arg, 2 Gly, Ile, 3 Leu, 2 Lys, 2 Met, 2 Phe, Pro, Ser, 2 Tyr, and Val.Treatment with Edman’s reagent releases PTH-Gly.Carboxypeptidase A releases Phe. Treatment with trypsin yields the following four peptides:1. Gly-Leu-Tyr-Phe-Lys 3. Val-Ile-Arg2. Ser-Met-Gly-Leu-Tyr-Lys 4. Met-Leu-Pro-Phe
Assign IUPAC names to each of the following small peptides.Gly-Ala-Leu Gly-Tyr-Ser-Ser
Draw the peptide ASK(ala-ser-lysine) with proper stereochemistry? what is its pI(isoelectric point)and why the structure is planar? Use the following table to look at pka's of the amino acids and solve this question.
An amino acid mixture consisting of lysine,leucine, and glutamic acid is to be separated by ion-exchangechromatography, using a cation-exchange resin at pH 3.5, with theeluting buffer at the same pH. Which of these amino acids will beeluted from the column first? Will any other treatment be neededto elute one of these amino acids from the column?
The peptide cys-leu-glu-ala-cys-lys is at pH 7 in oxidizing conditions. Part a) Draw the peptide's titration curve. Part b) What would the pI of the peptide be around?

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