Imagine that a cell contains a temperature-sensitive mutation such that nuclear lamins cannot be phosphorylated at the restrictive temperature. Describe the phenotype of the mutant cells during mitosis at the restrictive temperature. O The mutant cells will not be able to decondense chromosomes and will be arrested in prophase O The mutant cells will not be able to assemble the mitotic spindle and will be arrested in metaphase O The mutant cells will not be able to disassemble the nuclear envelope and will be arrested in prophase The mutant cells will go through multiple mitotic cycles without cytokinesis and will become multi-nucleated The mutant cells will be unable to reassemble the nuclear envelope and will be arrested in telophase

Imagine that a cell contains a temperature-sensitive mutation such that nuclear lamins cannot be phosphorylated at the restrictive temperature. Describe the phenotype of the mutant cells during mitosis at the restrictive temperature. O The mutant cells will not be able to decondense chromosomes and will be arrested in prophase O The mutant cells will not be able to assemble the mitotic spindle and will be arrested in metaphase O The mutant cells will not be able to disassemble the nuclear envelope and will be arrested in prophase The mutant cells will go through multiple mitotic cycles without cytokinesis and will become multi-nucleated The mutant cells will be unable to reassemble the nuclear envelope and will be arrested in telophase

Biochemistry

6th Edition

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter28: Dna Metabolism: Replication, Recombination, And Repair

Section: Chapter Questions

Problem 19P: Figure 28.11 depicts the eukaryotic cell cycle. Many cell types â€œexitâ€� the cell cycle and...

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Biologists have long been interested in the effects of radiation on cells. In one experiment, researchers examined the effect of radium on mitosis of chick embryo cells growing in culture. A population of experimental cells was examined under the microscope for the number of cells in telophase (as a measure of mitosis occurring) before, during, and after exposure to radium. The results are shown in the Figure. What is the effect of radium exposure on mitosis? Source: R. G. Canti and M. Donaldson. 1926. The effect of radium on mitosis in vitro. Proceedings of the Royal Society of London, Series B, Containing Papers of a Biological Character 100:413419.
Passage of cells from G1 through R to S depends on the interaction of various signal molecules, proteins and enzymes which regulate the cell cycle. Imagine I have just induced a mutation in a cell line which prevents the breakdown of the cyclin molecule we discussed. Which of the following events do you predict will happen? circle all that apply Select one or more: a. Uncontrolled cell replication may result b. Retinoblastoma protein will be continuously active c. The cells will be stuck in G1 phase d. The cells will begin to replicate their DNA e. Cyclin dependent kinase (Cdk) will be continuously active
The cell cycle of an average mammalian cell is illustrated below. How long did it take for the above-illustrated mammalian cell to complete one cell cycle?How long did the mitosis/cytokinesis phase (M phase) last? What % of the total cell-cycle timedoes that constitute to? What phase takes the longest time to complete during cell cycle and what % of the total cellcycle time does that constitute to? Explain why you think this might be the longest phase of thecell cycle.How long was the S phase? What is happening in the cell during the S phase?
Match the process with the correct phase of the cell cycle (G1, S, G2, M). If the process occurs in M phase indicate subphase (ex. telophase). A) ___________________ The bipolar spindle forms from microtubules. B) ___________________ The nucleus breaks down. C) ___________________ Mitochondria are duplicated. D) ___________________Sister chromatids are formed. E) ___________________Extra cell membrane is synthesized by the smooth ER. F) ___________________DNA is compacted into chromatin.
One approach to studying the regulation of cell cycle progression (particularly in an era when genetic and molecular biology manipulations were less readily accomplished in mammalian cells) was to use treatments that induced cells to fuse and then monitor the behavior of the two nuclei in the resulting cell. The figure below depicts data from one such study. The investigators did preliminary work to produce populations of cells that were synchronized in various stages of the cell cycle (G1, S, or G2 in the examples shown below). They then fused the cells in different combinations and monitored subsequent events in each of the nuclei. For purposes of this question, we will pay particular attention to what occurred in the nucleus that came from the cell in G1. In one experiment (I), cells in the G1 and S phases were fused. That event caused the nucleus from the G1 cell to very quickly enter the S phase (sooner than it would otherwise have done so). In contrast, in a second experiment…
One approach to studying the regulation of cell cycle progression (particularly in an era when genetic and molecular biology manipulations were less readily accomplished in mammalian cells) was to use treatments that induced cells to fuse and then monitor the behavior of the two nuclei in the resulting cell. The figure below depicts data from one such study. The investigators did preliminary work to produce populations of cells that were synchronized in various stages of the cell cycle (G1, S, or G2 in the examples shown below). They then fused the cells in different combinations and monitored subsequent events in each of the nuclei. For purposes of this question, we will pay particular attention to what occurred in the nucleus that came from the cell in G1. In one experiment (I), cells in the G1 and S phases were fused. That event caused the nucleus from the G1 cell to very quickly enter the S phase (sooner than it would otherwise have done so). In contrast, in a second experiment…
When studying living cells in a laboratory, researchers sometimesuse drugs as a way to make cells remain at a particular stage of thecell cycle. For example, aphidicolin is an antibiotic that inhibitsDNA synthesis in eukaryotic cells and causes them to remain inthe G1 phase because they cannot replicate their DNA. In whatphase of the cell cycle—G1, S, G2, prophase, metaphase, anaphase,or telophase—would you expect somatic cells to stay if they weretreated with each of the following types of drug?A. A drug that inhibits microtubule formationB. A drug that allows microtubules to form but prevents them fromshorteningC. A drug that inhibits cytokinesisD. A drug that prevents chromosomal condensation
Need help If the mitotic spindle does not correctly attach to chromosome kinetochores, the spindle assembly checkpoint will be activated. Select all correct outcomes for chromosomes not correctly attached to mitotic spindle A) centrosome duplication will not occur B) M cyclin will be degraded C) APC will be inhibited D) chromosomes will not segregate E) Mad2 will bind to unattached kinetochores F) mitosis will halt at metaphase/anaphase transition
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In class, we talked about flow cytometry which allows scientists to determine what stage of the cell cycle cells are in by measuring their DNA content. Draw what the graph would look like for the mutant below at a 0-time point when there are 1000 cells total, with 5000 cells in G1, 350 cells in M and G2, and 150 cells in S phase. And then a graph of 1 hour later when the cells should have competed for mitosis: → loss of function of cdc25
What occurs during metaphase phase of mitosis? A. Microtubules can be lengthened by adding subunits at the + end to move chromosomes towards the metaphase plate B. Microtubules can be shortened to move chromosomes towards the metaphase plate. C. Motor proteins pull chromosomes along the microtubules to move them towards the metaphase plate. D. All of the above E. None of the above
What is the major difference between M phase and interphase of the cell cycle? We can see activity within the cell only during M phase. Interphase is when the cell divides, whereas M phase is where it is mute. We can see activity within the cell only during interphase. M phase involves color changes, which is why there are chromosomes.