In a biochemistry laboratory, you are asked to prepare a buffer solution to be used as a solvent for isolation of an enzyme. On the shelf are the following solutions, all 1.00 M: formic acid (Ka = 1.8 × 10-4), acetic acid (Ka = 1.8 × 10-5), sodium formate (NaHCO2), and sodium acetate (NaCH3CO2). Describe how you would prepare 1.0L of a pH = 4.56 buffer solution with total molarity of 0.20 M. Mix together i g of sodium acetate i Lof 1.0 M acetic acid acid, and enough water to make 1.0 L.

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In a biochemistry laboratory, you are asked to prepare a buffer solution to be used as a solvent for isolation of an enzyme. On the shelf
are the following solutions, all 1.00 M: formic acid (Ka = 1.8 × 10-4), acetic acid (Ka = 1.8 × 10-5), sodium formate (NaHCO2), and sodium
acetate (NaCH3CO2). Describe how you would prepare 1.0L of a pH = 4.56 buffer solution with total molarity of 0.20 M.
Mix together i
g of
sodium acetate
i
Lof 1.0 M
acetic acid
acid, and enough water to make 1.0 L.
Transcribed Image Text:In a biochemistry laboratory, you are asked to prepare a buffer solution to be used as a solvent for isolation of an enzyme. On the shelf are the following solutions, all 1.00 M: formic acid (Ka = 1.8 × 10-4), acetic acid (Ka = 1.8 × 10-5), sodium formate (NaHCO2), and sodium acetate (NaCH3CO2). Describe how you would prepare 1.0L of a pH = 4.56 buffer solution with total molarity of 0.20 M. Mix together i g of sodium acetate i Lof 1.0 M acetic acid acid, and enough water to make 1.0 L.
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