In a surfactant molecule the active head surface area was 70 (secured angstroms) whereas it is hydrocarbon chain length and volume were 20 (angstroms) and 50 (cubic angstroms) respectively. The expected micellar shape is then?. Select one O a Spherical Ob. Rod-like O c. lamellar d None
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- Please enumerate and explain 1. WHAT ARE THE GENERAL RULES IN GRAMS STAINING CLASSIFICATION OF BACTERIA?Mention the relative sizes of hard gelatin capsule with their corresponding volume and fill weight at 0.6g/cm3 powder densityWhich are true for polarography? I Polarography is amperometry conducted with a dropping Hg electrode. II The diffusion current in the plateau region of the polarographic wave is proportional to the concentration of the analyte and is used for quantitative analysis. III The half-wave potential for maximum current is characteristic of a given analyte in a given medium and can be used for qualitative analysis for the analyte. IV Fresh Hg drops give reproducible results. V Polarography is used to primarily to study oxidations Hg may reduce when studying reductions. A.I, III, and IV B. II, III, and V C. I and V D. III, IV, and V E. II, III, and IV
- In HPLC, why is purging being done before analysis of samples. Outline the procedure on how purging is performed.A student performs a separation by TLC on a silica gel platee that results in three spots. A red spot is measured 23 mm away from the original sample spot, a blue spot is 42 mm away, and a yellow spot is 49 mm away. the distance from the original spot to where the solvent stopped is 55 mm. a) calculate the Rf value for each componenet in the mixture (show work and report th correct number of significant figures) b) the silica gel plate is polar. rank the three componenets in the mixture from lowest to highest polarity. Explain your answer.Pick all that are TRUE regarding analysis of quaternary structures of proteins using polyacrylamide electrophoresis:I. The added β-mercaptoethanol disrupts S--S bonds bridging the polypeptide chains causing the appearance of higher Rf bands compared to the native protein run. II. Heating up any protein before subjecting to SDS-PAGE will always result in the formation of more than one band.III. A good asymmetrical gel layout would be : (Lane 1) MW ladder, (2) native protein, (3) protein + β-ME, (4) protein + HCL, (5) protein + β-ME + HCl.IV. Formation of a single band in the protein + β-ME + HCl run, whose Rf is lower than the native run, could be indicative that the protein is a homodimer.A. I onlyB. I and IIC. II and IIVD. None is true.pls explain
- How can false low results due to the Hook Effect be accounted for in immunoassay testing?A tissue plasminogen activator (T PA) opthalmic solution is prepared to contain 25 mg / 100 mL. A.Calculate the percent concentration of TPA in the solution. B.What volume of a solution containing TPA , 50 mg/ 50 mL , should be used to prepare each 100 mL of opthalmic solution?Glass is a notorious source of metal ion contamination. Three glass bottles were crushed and sieved to collect 1-mm pieces.8 To see how much Al31 could be extracted, 200 mL of a 0.05 M solution of the metal-binding compound EDTA were stirred with 0.50 g of ,1-mm glass particles in a polyethylene flask. The Al content of the solution after 2 months was 5.2 mM. The total Al content of the glass, measured after completely dissolving some glass in 48 wt% HF with microwave heating, was 0.80 wt%. What fraction of the Al was extracted from glass by EDTA?
- 2 d) In a typical HPLC column, 150 atm of pressure is required to push the mobile phase through the column. Why is high pressure needed in HPLC? e) Why are HPLC particles porous?A batch of raw milk was tested and found to contain a bacterial population of 8xl06cfu/ml. It is to be pasteurised at noc for 33 seconds. The average D value at 52°C for the mixed population is 11 min. The z value is 10°C.i)Explain the terms "D value" and "z value".ii)Calculate how many organisms will be left after pasteurisation.iii)Hpw much time will be needed at 62°C to accomplish the same degree oflethality?iv. How much time will be needed at 82°C to achieve the same degree oflethality?A hi Experts...please help me, i need it until 6:30 pm. Hope you can answer it BEFORE the time thank you soo much! KINDLY MAKE THE ANSWER SHORT FOR 2-3 SENTENCES IF POSSIBLE. ---Explain the effect of heat and time on foaming characteristics of protein