In HPLC, why is purging being done before analysis of samples. Outline the procedure on how purging is performed.

Q: Explain the principle of working and operation of Dye Penetration Technique Inspection with neat…

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Q: What is deionized water? What kind of impurities are not removed by deionization?

A: Purified water does not contain any impurity. There are various methods for purification of water…

Q: In an experiment that uses TLC, like the one performed in this course, which of the following…

A: Thin layer chromatography (TLC) is used to isolate and separate non-volatile mixtures based on their…

Q: 1. How does sodium 2-phenylphenolate react with acid and/or base? Draw the acid-base reaction (with…

A: This is a normal acid base reaction in which phenolate is the base and that of H+ is acid, so base…

Q: For standard turbidity measurements, the increase in signal is due to a.) higher amounts of analytes…

A: Its intensity ( scattered light ) can also be measured by this, Precipitate size and shape are…

Q: What is 2D gel electrophoresis

A: 2D gel electrophoresis is a technique for detection and separation of proteins. It is a combination…

Q: Which statement is true?   Statement 1: In a paper chromatography that uses a cellulose-based…

A: Given : Two statements are given  To find : True statements  Solution : Chromatography is a…

Q: 10. Consider the following compounds when answering the questions below. Assume these compounds can…

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Q: Spinach chromatography lab: provide a short description of the following processes: a) Spinach…

A: Column chromatography is an isolation and purification technique used extensively to obtain…

Q: Why is catalytic dewaxing not considered as a separation process?

A: Since Catalytic dewaxing involves breaking and making bonds, so it is usually referred to as a…

Q: a) Discuss the effect of solvent polarity on Rf-values. b)Explain how the Rf (Retention…

A: Since you have posted two questions, we will solve first question for you. Please repost other…

Q: Why do we need to add sand on top of the silica in column chromatography? What could happen if we…

A: The presence of different compounds in the mixture is separated by employing the separation methods.…

Q: Find the Rf value from given data. Thank you

A: In Thin Layer Chromatography, the compounds can be separated by the solvents in the TLC plate. The…

Q: Consider stereoisomers. What type of isomers would you expect would and what types could not be…

A: Thin-layer chromatography can be used to sperate cis and trans-isomer because these isomers have…

Q: What further testing should be done to extract and compare the composition of food dyes used In two…

A: Food coloring or the additives refers to a dye or a pigment or any substance which  imparts color to…

Q: What is in a fractionating column?     Silica gel     Collected fractions     Glass or plastic…

A: Using fundamental of fraction distillation process and hardware.

Q: For GC analysis, the following can cause band broadening and poor resolution OUse of automatic…

A: Chromatography is separating technique for mixtures into it's components. It is based on the…

Q: 2. In HPLC analysis, why Would peak area is preferred to peak height in evaluating the caffeine…

A: Since you have asked multiple questions, we will answer the first question for you. To get the…

Q: Three sample, X (neutral), Y (basic), and Z (acidic), were mixed and dissolved together in…

A: Dissolve the mixture in dichloromethane and separate the organic and aqueous layer using liquid…

Q: If 15 mL of dichloromethane was used at one time for extraction, will the amount of the substance…

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Q: Why sometimes derivatization is used in the sample preparation for GC-MS?

A: The role of derivatization in GC-MS is given below

Q: 10)Examine each of the errors below, discuss how the error would affect the separation. Describe how…

A: Hello. Since the question contains more than three sub-parts, the first three sub-parts shall be…

Q: In a liquid - liquid extraction, what is preferable? Three extractions with a small volume or one…

A: Liquid-liquid extraction is also known as solvent extraction or solvent partitioning. In this…

Q: Analysis of protein purity using two-dimensional electrophoresis could involve

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Q: The following Rf values were computed from a normal phase chromatography experiment. Arrange them…

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Q: What adsorbents can be used to remove impurities from benzoic acid? Explain the molecular basis for…

A: Benzoic acid is an organic compound. Therefore, the impurities that remain dissolved in benzoic acid…

Q: Describe how you would prepare a sample for TGA analysis if it were provided in the form of: (i)…

A: Thermogravimetric analysis (TGA) of polymers is conducted to measure weight changes as a function of…

Q: please answer hurry up ..... What kind of “cation and anion exchanger resins” can be used in the…

A: Ion exchange chromatography useful for separation of ionic compounds. Separation is based on…

Q: 1. You know that these types of compounds can be analyzed by either GC or HPLC but you are told by…

A: GC can only apply to  analyse volatile substance while HPLC can handle any soluble compound…

Q: What are your observations performing paper chromatography

A: Paper chromatography is of two types . Thin layer chromatography Preparative TLC

Q: Why does the mobile phase in reversed phase (C18) HPLC needs to be acidic?

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Q: Question a) Discuss the effect of solvent polarity on Rf-values.

A: Since you have posted two questions, we will solve first question for you. Please repost other…

Q: If you were given a soil sample to analyze for its levels of herbicide, 2,4-D, escribe how you would…

A: 2 4 dichlorophenoxyacetic acid , is a herbicide that's a serious threat to environment.

Q: How does ammonia, dimethylglyoxime (DMG), and 8-hydroxyquinoline (8HQ) interact with your cations,…

A: In paper chromatography, substances are distributed between a stationary phase and a mobile phase.…

Q: Many ink dyes can be separated by the technique of___________ chromatography

A: Thin Layer Chromatography is a method for separating non-volatile substances. The separation…

Q: Briefly explain how cation exchange chromatography can used in protein separation?

A: Cation exchange chromatography is a form of ion exchange chromatography (IEX), which is basically…

Q: TGA analysis

A: Ans. Thermogravimetric analysis or TGA techniques helps in determination of thermal stability of…

Q: Z and E -oct-3-en-2-ol is hard or easy  to separate in thin layers in chromatography?

A: Greater the polarity difference between the two components of a mixture easier will be the…

Q: 3. Compound A has an Rf of 0.75 and compound B has an Rf of 0.35. Which compound will elute first…

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Q: Compare the column material of GC and LC in terms of the “functional groups” taking place on the…

A: In GC there are two type of coloumn.packed coloumn And capillary Coloumn. In gas liquid…

Q: Why is it required to add drying agent like sodium sulphate to the organic layer which may contain…

A: In an organic solution, there are often traces of water present because water is slightly soluble in…

Q: a) What is capillary electrophoresis used for? b) Describe electrophoresis. c) Describe…

A: Since you have posted multipart of question as per the guidelines i have solved first three subparts…

Q: When would using Thin Layer Chromotography (TLC) NOT be useful in conducting an oxidation reaction?…

A: Although it is a very simple and convenient technique, one of its limitations is that it cannot tell…

Q: The pure samples, R & S-Carvone and will undergo which analyses below (choose all that are…

A: Since you have asked multiple question, we will solve the first question for you. If you want any…

Q: why do we have to order samples (from least concentrated -most concentrated) when measuring the…

A: A question based on qualitative analysis, which is to be accomplished.