In another experiment using Quikchange to amplify the pQE.1-CRYGD plasmid containing our mutation, you added all needed components properly including primers and template DNA, but your Quikchange still doesn't work. One change was made to the protocol in step 1, you performed your reaction at 50 degrees C instead of 95 degrees C. Why didn't the reaction work? In the next attempt, you add dNTPs, polymerase, buffer, and your primers, but forgot to add your template DNA. Why did you know that the reaction could not work without template DNA?

In another experiment using Quikchange to amplify the pQE.1-CRYGD plasmid containing our mutation, you added all needed components properly including primers and template DNA, but your Quikchange still doesn't work. One change was made to the protocol in step 1, you performed your reaction at 50 degrees C instead of 95 degrees C. Why didn't the reaction work? In the next attempt, you add dNTPs, polymerase, buffer, and your primers, but forgot to add your template DNA. Why did you know that the reaction could not work without template DNA?

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter13: An Introduction To Genetic Technology

Section: Chapter Questions

Problem 20QP: Analyzing Cloned Sequences A base change (A to T) is the mutational event that created the mutant...

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