8In oxidative phosphorylation, a chemical agent that prevents the enzymatic activity of F1 woulddirectly [1], whereas a chemical agent that inhibits interaction of ubiquinone with Fe-S centreswould directly___[2].Select one:1 = Uncouple phosphorylation from electron transport; 2 = Inhibit ATP synthesisO b. 1 = Inhibit ATP synthesis; 2 = Uncouple phosphorylation from electron transportC. 1 = Inhibit electron transfer; 2 = Inhibit ATP synthesisd. 1 = Inhibit ATP synthesis; 2 = Inhibit electron transferOOOReport question issueNote

The correct answer is:c. 1 = Inhibit electron transfer; 2 = Inhibit ATP synthesis

Answered: In oxidative phosphorylation, a…

Biochemistry

6th Edition

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter20: Electron Transport And Oxidative Phosphorylation

Section: Chapter Questions

Problem 19P

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Imagine that you are working with isolated mitochondria and you manage to double the ratio of protons outside to protons inside. In order to maintain the overall Gat its original value (whatever it is), how would you have to change the mitochondria membrane potential?
pH and in the Chloroplast Proton-Motive Force (Integrates with Chapter 20.) In mitochondria, the membrane potential () contributes relatively more to p (proton-motive force) than does the pH gradient (pH). The reverse is true in chloroplasts. Why do you suppose that the proton-motive force in chloroplasts can depend more on pH than mitochondria can? Why is () less in chloroplasts than in mitochondria?
5. a) The cell creates molecules of NADH and FADH2to use in the electron transport chain as they are electron carrier molecules. The electron transfers from these moleculesdrives the movement of what molecule across the mitochondrial membrane?b) What is chemiosmosis?c) How does the cell use chemiosmosis to drive overcome the thermodynamic barrier of bringing phosphates close togehter to create ATP?d) What steps of oxidative phosphorylation are in the cytosol and which are in the mitochondria?e) Cellular respiration can be controlled at the post-translational level. What does this mean, using GLUT as an example? Why is post-translational control of GLUT evolutionarilyadaptive over transcriptional control?f) How does establishment of equillibria of various molecules control metabolic reactions? What are three ways high ATP in the cell control cellular respiration?g) Why are the enzymes controlling the initial steps of various biochemical pathways are targeted by allosteric control?
If actively respiring mitochondria are exposed to an inhibitor of ATPADP translocase, the electrontransport chain ceases to operate. Why?
A suspension of inside-out submitochondrial particles (i.e., the F1 unit is on the outside of the particle) is placed in a solution that contains ADP, Pi, and NADH. Will increasing the proton concentration of the solution result in ATP synthesis? Explain.
1) When a de-coupler such as 2,4-DNP is added to a respiring mitochondrion, which of the following effects on the process of ETC-OP will be true, which will be false. a) Mitochondria no longer reduce O2 to H2 b) Electrons no longer enter the electron transport chain. c) Activity of Complex V (ATP Synthase) is greatly decreased. d) The P/O ratio for FADH2 becomes larger than the P/O ratio for NADH. e) Protons are no longer pumped from the mitochondrial matrix to the intermembrane space. 2) The rate-limiting step in fatty acid synthesis is: a) condensation of acetyl-CoA and malonyl-CoA. b) formation of acetyl-CoA from acetate. c) formation of malonyl-CoA from malonate and coenzyme A. d) the reaction catalyzed by acetyl-CoA carboxylase.
6. The lens of the eye si the light refracting medium of the eye, and ti has no mitochondria. Glucose is used sa an energy source ni the lens. Which metabolic pathway of glucose supplies energy to the crystalline eye lens? To answer the question: a) write a diagram of the metabolic pathway that provides the eye's lens with ATP, specify enzymes and coenzymes; b) mark the reactions associated with the ATP consumption and synthesis, calculate the ATP yield during the process; )c specify the mechanisms ofATP synthesis ni the process; d)name the tissues and cells in which the ATP synthesis is the same as in hte lens; e) writethe dehydrogenation reaction and the reactionoffinal product formation occurring in the process;
please draw1 10. Diagram in detail the oxidative phosphorylation stage of cellular respiration demonstrating how the bulk of the ATP is made during that stage. Accompanying your diagram should be a paragraph explaining the ATP production process
1)Where is the most Potential Energy Stored at the end of the Citric Acid Cycle? A. High energy electron NADH B. Electron Tansport chain C. G3P D. ATP molecuoles 2) The movement of several solutes simoultanously a. Simple diffusion b. bulk transport c. Passive trasnsport d. Active Trasnport E. Osmosis 3) What is the source of electrons for photosysthesis? a. Oxygen b. ATP c. water D. Glucose E. Carbon dioxide 3) Movement of several solute acrossss cocentration graidients A. Simple diffusion B. Bulk transpoert C. Passive trasnport D. Active Transport E. Facilitated diffusion
Converting 1 mol of glucose to 2 mol of oxaloacetate will directly net __ ATP/GTP, __ NADH + H + , __ FADH2? .
Luckily, protons (a.k.a. hydrogen ions or H+) are small enough to passively go right through a membrane, otherwise mitochondria would have to work too hard to regenerate ATP! True or False?
What effect will increasing the number of c subunits in ATP synthase have on the P:O ratio? A. It will increase for both NADH and FADH2. B. It will increase for NADH and decrease for FADH2. C. It will decrease for NADH and increase for FADH2. D. It will decrease for both NADH and FADH2. E. It will remain unchanged for both NADH and FADH2.

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