N-(2-hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid) buffer at pH 7 with 400 mM NaCl. A A purified protein is in a Hepes dialysis membrane tube holds a 5.0 mL. sample of the protein solution. The sample tube floats in a beaker containing 0.50 L of the same Hepes buffer, but with 0 mM NaCl, for dialysis. Small molecules and ions (such as Na*, CI, and Hepes) can to diffuse across the dialysis membrane, but the protein cannot. Assume there are no sample volume changes during the dialysis. Calculate the final concentration of NaCl in the protein sample once the dialysis has come to equilibrium. Calculate the final NaCl concentration in the 5.0 mL protein sample after dialysis in 300 mL of the same Hepes buffer, with 0 mM NaCl, twice in succession. [NaCl] after a single dialysis: [NaCl] after a double dialysis: mM mM
N-(2-hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid) buffer at pH 7 with 400 mM NaCl. A A purified protein is in a Hepes dialysis membrane tube holds a 5.0 mL. sample of the protein solution. The sample tube floats in a beaker containing 0.50 L of the same Hepes buffer, but with 0 mM NaCl, for dialysis. Small molecules and ions (such as Na*, CI, and Hepes) can to diffuse across the dialysis membrane, but the protein cannot. Assume there are no sample volume changes during the dialysis. Calculate the final concentration of NaCl in the protein sample once the dialysis has come to equilibrium. Calculate the final NaCl concentration in the 5.0 mL protein sample after dialysis in 300 mL of the same Hepes buffer, with 0 mM NaCl, twice in succession. [NaCl] after a single dialysis: [NaCl] after a double dialysis: mM mM
Chapter10: Reconstitution Of Powdered Drugs
Section: Chapter Questions
Problem 5.3P
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![N-(2-hydroxyethyl)piperazine-N'-(2-ethanesulfonic
acid) buffer at pH 7 with 400 mM NaCl. A
A purified protein is in a Hepes
dialysis membrane tube holds a 5.0 mL sample of the protein solution. The sample tube floats in a beaker containing 0.50 L of
the same Hepes buffer, but with 0 mM NaCl, for dialysis. Small molecules and ions (such as Na*, CI", and Hepes) can to diffuse
across the dialysis membrane, but the protein cannot. Assume there are no sample volume changes during the dialysis.
Calculate the final concentration of NaCl in the protein
sample once the dialysis has come to equilibrium.
Calculate the final NaCl concentration in the 5.0 mL protein
sample after dialysis in 300 mL of the same Hepes buffer,
with 0 mM NaCl, twice in succession.
[NaCl] after a single dialysis:
[NaCl] after a double dialysis:
mM
mM](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fa7be6961-269b-4114-8e6f-9ec505405bc0%2F2aa74db6-36bb-40c5-9449-10ad9beb269c%2Fafemr9k_processed.jpeg&w=3840&q=75)
Transcribed Image Text:N-(2-hydroxyethyl)piperazine-N'-(2-ethanesulfonic
acid) buffer at pH 7 with 400 mM NaCl. A
A purified protein is in a Hepes
dialysis membrane tube holds a 5.0 mL sample of the protein solution. The sample tube floats in a beaker containing 0.50 L of
the same Hepes buffer, but with 0 mM NaCl, for dialysis. Small molecules and ions (such as Na*, CI", and Hepes) can to diffuse
across the dialysis membrane, but the protein cannot. Assume there are no sample volume changes during the dialysis.
Calculate the final concentration of NaCl in the protein
sample once the dialysis has come to equilibrium.
Calculate the final NaCl concentration in the 5.0 mL protein
sample after dialysis in 300 mL of the same Hepes buffer,
with 0 mM NaCl, twice in succession.
[NaCl] after a single dialysis:
[NaCl] after a double dialysis:
mM
mM
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