In TLC chromatography there is a technique known as Co-spotting. In this technique, a sample of an unknown substance is charged at the same point, together with a known substance and stimulated by some kind of stimulant. In the example below, we loaded biphenyl (a known substance) together with two unknown substances A and B. Is A, or B biphenyl? Justify your answer. What is the alternative method to co-spotting, and which one do you think is better?

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