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- Aside from TLC and paper chromatography, what are the other types of chromatography. Discuss their principles, applications and limitations.A sample of fried potatoes weighing 200 g was extracted using a volatile organicsolvent. The recovered cooking oil weighed 15 g. What methods from thisexperiment could be used to separate the cooking oil after the extraction? (evaporation, gravity filtration or vacuum filtration?) why so?Name 3 adsorbents used in TLC. Mention their use and properties. Why is it important to use a pencil, and not pen, for marking TLC plates?
- Which of the following are necessary steps in performing paper chromatography? I. Saturate the chamber with the vapor of the mobile phase II. Allow the chromatogram to touch the sides of the beaker III. Place the spots at the same height as the solvents IV. Spot the samples 2 cm apart from each other a. I and IIIb. I and IVc. I, III and IVd. II, III and IVWhich of the following chromatographic methods cannot use a mixture of liquid solvents (eluents) during the separation process? Thin layer chromatography High pressure liquid chromatography Column chromatography Gas chromatographyWhich is more accurate, a transfer pipet or a measuring pipet? What is theuncertainty in microliters when you deliver either 10 mL or 100 mL from a 100-mL adjustable micropipet?
- 2. a) What is a bonded phase in HPLC?b) Give an example of a bonded phase in reversed-phase liquid chromatography and normal phase liquid chromatography.c) Describe when you would choose to use a reversed-phase column or a normal-phase column for an analysis of a mixture.What types of flow meters are used in gas chromatography?Which of the following is NOT correctly related to the parts of paper chromatography set-up? a. Mobile phase: solvent b. Solute/Mixture: extract c. Stationary phase: paper d. Chromatographic chamber: sealed container
- Use a chemical dictionary, chemical text or encyclopedia to find a specific definition of "chromatography." List a few mobile and stationary phases.Which of the following could be a consequence of an open and uncovered chromatographic chamber? I. The travel rate of a component would be faster. II. The chromatographic chamber will not be saturated. III. The mobile phase will take a longer time to reach the end line. Select one: a. I, II, and III b. I only c. I and III d. II and IIIA piece of filter paper is placed in the TLC developing chamber to: Choose one: provide a lining for the TLC plate to rest on. help the chamber saturate with the developing solvent's vapor. make it easier to visualize the solvent front level. protect the TLC plate.