In your own words, describe the series of steps necessary to clone a gene.

Q: Explain why exome sequencing can be almost as valuable as genome sequencing.

A: Whole genome sequencing refers to technique which used to analyze complete genome of an organism at…

Q: Why is genome database and annotation relevant to Genome sequencing?   Are there any examples?

A: Genome: the set of genes. Genome database: it is the collection of all information on the human…

Q: Describe the shotgun method for sequencing an entire genome.

A: Genome or genetic material of a cell is the DNA content present in the nucleus. DNA or…

Q: How could the information from sequencing your genome be used against you theoretically if it fell…

A: Genome sequencing is the most powerful key to get the entire information regarding the specimen.  It…

Q: Why can’t all genetic diseases be treated with gene therapy ? Explain how the ideal procedure for…

A: Gene therapy is one of the treatment methods to treat genetic diseases. It involves the replacement…

Q: In any transformation experiment involving any gene of interest, what is it you are selecting for?…

A: Transformation is the genetic alteration of a cell resulting from the direct uptake and…

Q: What is the principle of next generation sequencing?

A: The process of determining the nucleic acid sequence is known as DNA sequencing.

Q: When you know the sequence of the gene for the ocular protein where would you get the DNA you need…

A: The cloning is the method of recombinant DNA technology in which a piece of known DNA sequence is…

Q: How does gene cloning produce many copies of a gene?

A: Gene is a functional unit of heredity. A gene is a sequence of nucleotides in genome that codes for…

Q: Why Gene-prediction programs are used ?

A: In a DNA sequence, where those codons might fall even where a functional protein might be present or…

Q: Describe step 1: hybridization of template and primer in automated sanger sequencing

A: Introduction  Sanger sequencing is also known as the “chain termination method”. It is a method for…

Q: Discuss the concept of the use of DNA in genome identification.

A: DNA (Deoxyribo nucleic acid) is a double stranded genetic element in all prokaryotic and eukaryotic…

Q: Why is genome sequencing important?

A: Genome sequencing is a laboratory technique in which the order of all the nucleotides in an…

Q: Describe the process of molecular cloning

A: The term Cloning refers to production of tangible copy. Molecular cloning is additionally called as…

Q: What Does It Mean “To Clone”?

A: DNA(deoxyribonucleic acid) is a double-stranded helical genetic material containing thousands of…

Q: What is exome sequencing ? Why it is important ?

A: The exome has historically been described as the sequence in the genome that includes all exons of…

Q: How the Sequencing Technologies Have Progressed Rapidly ? Explain about this ?

A: Early efforts at sequencing genes ere troublesome, when Gilbert and Maxam reported the sequence of…

Q: What is the point of genome sequencing?

A: DNA is the main constituent of the chromosome. It contains all information about protein that forms…

Q: What is RNA sequencing ? Why it is important ?

A: The biochemical material that is carried from the preceding generation to the succeeding generation…

Q: describe the main steps required to clone a gene and produce a protein.

A: Gene cloning is the technique of locating and copying (cloning) a gene of interest from all the DNA…

Q: Briefly explain how synthetic probes are created to screen a DNA library when the protein encoded by…

A: Synthetic probe is fragment of DNA. to screen a DNA library RNA of variable length approximately 100…

Q: process of overlap assembly in whole genome sequencing

A: Genome sequencing Genome sequencing or gene sequencing is a way of arranging nucleotides the n the…

Q: Explain the Several Limitations Positional Cloning Has?

A: Cloning is the process of making exact copies of the parental organisms with genetic and phenotypic…

Q: What is the difference between a cell strain, a cell line, and a clone?

A: All living organisms are made up of highly organized structures known as cells and the cells are…

Q: What are the advantages of Next Generation Sequencing?

A: DNA sequencing is a biochemical method for determining the order of nucleotide bases, A, G, C, and…

Q: Give a step by step method and diagram of how reversible terminator sequencing works.

A: The reversible terminator sequencing or Illumina dye sequencing is a technique used to determine the…

Q: Describe the outcome of a chain-terminator sequencing procedure in which (a) too little ddNTP is…

A: Sanger DNA sequencing is also known as the sequencing process of chain-termination. In addition to…

Q: Explain the process of genome editing ?

A: Introduction:- The genome is a cell's complete set of genetic instructions. The genome of humans is…

Q: You may have heard of Dolly, the cloned sheep grown from an embryo created in a laboratory. But in…

A: Dolly is the sheep, which is considered as the first genetically modified (GMO) organism, which was…

Q: What are the main  differences between whole genome sequencing and whole exome sequencing?

A: whole genome sequencing is sequencing the entire genome of the organism, where as whole exome…

Q: Define molecular cloning

A: The DNA molecules that are formed by laboratory methods involving genetic recombination is called…

Q: What is Sanger sequencing? Why do we use ddNTP? How to read a DNA sequence gel? c. What is a cDNA…

A: Your question contains multiple sub-parts, and as per our policy, we only answer the first three…

Q: Explain about concept of synthetic genomes ?

A: One goal of synthetic genomics and synthetic biology is to create new creatures and biological…

Q: Explain in detail types of DNA sequencing?

A: DNA sequencing is a technique which is used to determine the sequence of DNA nucleotides within the…

Q: Why is RNA sequencing important?

A: RNA or ribonucleic acid is a polymer of ribonucleotides connected together via a phosphodiester…

Q: Explain how to determine whether a given clone contains all or only part of the gene ?

A: Gene cloning is described as the process that includes a relocation of gene of interest and which is…

Q: Explain how Sanger sequencing works

A: Genome sequencing: genome means total gene content present in the organism and sequence means to…

Q: In PCR, the goal is to make copies of?

A: The field of biology concerned with the molecular foundation of biological activity in and between…

Q: Explain why a cloning vector usually includes a selectable marker.

A: A cloning vector is a small piece of DNA that can be stably maintained in an organism, and into…

Q: "Whole-Genome Sequencing Is Widely Used for Sequencing and Assembling Entire Genomes". Explain this…

A: Whole genome sequencing It reveals an organism's complete DNA make-up ( allowing us to better…

Q: What is DNA cloning? What is the common organism that the scientists use to do cloning (explain…

A: DNA cloning is a technique of creating many copies of specific DNA that are an exact replica of…

Q: "The Quest to Create a Synthetic Human Genome" Define this ?

A: A genome is the complete set of genes or chromosomes present in the cells of an organism. A gene is…

Q: Explain why exome sequencing can be almost as valuable as genome sequencing. (Explain in your own…

A: Exome sequencing is one of the molecular tool to analyze the genes encoding for protein in entire…

Q: Let’s say, you want to deliver a gene into a cell and in your lab, there are lot of options…

A: Gene transfer: Method or process by which a gene is transferred from one DNA molecule to another.…

Q: What is the difference between manipulating a gene for gene therapy and for producing a transgenic…

A: The utilization of artificial tools and techniques to alter the genetic material of cells or…

Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.