Introducing the recombinant lac operon/mouse tyrosinase DNA into mice is only half of the equation for regulating tyrosinase. In addition to lac operon/mouse tyrosinase DNA, what other gene must be added to mice to regulate tyrosinase expression? the lacy gene the lac operator the lacl gene the lacZ gene

Biochemistry
6th Edition
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Reginald H. Garrett, Charles M. Grisham
Chapter29: Transcription And The Regulation Of Gene Expression
Section: Chapter Questions
Problem 8P: Alternative Splicing Possibilities Suppose exon 17 were deleted from the fast skeletal muscle...
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hext two questions.
The lac operon is used in countless ways for genetics research and biotechnology.
One important use of the lac operon is to regulate the expression of cloned genes. In
this application, some regulatory elements of the operon are fused to the cloned
gene that is to be regulated. One application of such technology was reported by
Cronin et al., The lac operator-repressor system is functional in the mouse, Genes
and Development 15 (2001):1506-17. These investigators fused a portion of the lac
operon to the mouse tyrosinase gene, a gene required for pigment production. This
recombinant DNA, composed of E. coli lac operon sequences and the mouse
tyrosinase gene, was introduced into albino mice using techniques described in
Chapter 20. Using the lac operon regulatory sequences, the investigators were able
to regulate tyrosinase gene expression. In fact, they were able to convert albino
(white) mice into brown mice. The following questions ask you to use your
knowledge of the lac operon to deduce how Cronin et al. were able to regulate
tyrosinase gene expression in mice.
Transcribed Image Text:hext two questions. The lac operon is used in countless ways for genetics research and biotechnology. One important use of the lac operon is to regulate the expression of cloned genes. In this application, some regulatory elements of the operon are fused to the cloned gene that is to be regulated. One application of such technology was reported by Cronin et al., The lac operator-repressor system is functional in the mouse, Genes and Development 15 (2001):1506-17. These investigators fused a portion of the lac operon to the mouse tyrosinase gene, a gene required for pigment production. This recombinant DNA, composed of E. coli lac operon sequences and the mouse tyrosinase gene, was introduced into albino mice using techniques described in Chapter 20. Using the lac operon regulatory sequences, the investigators were able to regulate tyrosinase gene expression. In fact, they were able to convert albino (white) mice into brown mice. The following questions ask you to use your knowledge of the lac operon to deduce how Cronin et al. were able to regulate tyrosinase gene expression in mice.
Introducing the recombinant lac operon/mouse tyrosinase DNA into mice is only half
of the equation for regulating tyrosinase. In addition to lac operon/mouse tyrosinase
DNA, what other gene must be added to mice to regulate tyrosinase expression?
the lacy gene
the lac operator
the lacl gene
the lacZ gene
4
Transcribed Image Text:Introducing the recombinant lac operon/mouse tyrosinase DNA into mice is only half of the equation for regulating tyrosinase. In addition to lac operon/mouse tyrosinase DNA, what other gene must be added to mice to regulate tyrosinase expression? the lacy gene the lac operator the lacl gene the lacZ gene 4
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