Q: The first step in identifying an organism from a pure working culture is to perform a(n) __________…
A: The pure culture consists of a single microorganism. This culture helps to easily evaluate the…
Q: What is the function of the mordant used in the Gram staining procedure? If the iodine step is…
A: Gram stain or Gram staining, likewise called Gram's strategy, is a technique for staining used to…
Q: Why is it important to limit the quantity of cells used to prepare a smear?
A: A thin layer of cells or tissues that are taken from the body of an organism under study which is…
Q: layer
A: The crystal violet eventually escapes from cytoplasm of the cell, if left too long for…
Q: n in detail gram stain biochemistry, procedures, gram-positive and gram negative cells.
A: Gram stain, a wide used microbiological, biochemical staining technique that greatly aids in the…
Q: Describe the Gram staining procedure
A: Gram staining is used to differentiate between Gram positive and Gram negative bacteria. These…
Q: What are the pros and cons of using the TCE stain-free method versus a traditional gel staining…
A: PAGE (polyacrylamide gel electrophoresis) is a well-known method for separating, detecting, and…
Q: Describe the bacteria observed in different staining procedures. Please refer to the photo -…
A: Bacteria can be stained by a variety of stains so that they can be visualised in contrast to the the…
Q: Is heat fixation used on bacterial smears that will undergo a capsule stain or flagella stain…
A: In capsule staining procedure “we do not heat fix and rinse the smear with water” as heat and water…
Q: What color would gram positive cells appear if ghr safranin staining step was left out of staining…
A: Gram staining is a technique used for differentiating the bacteria based on their cell wall…
Q: Move the images of bacterial cells into the chart to illustrate the color of gram-positive and…
A: Gram staining is a technique used to differentiate between two groups of bacteria namely…
Q: Are the structural differences between the cell walls of Gram-positive and Gram-negative bacteria…
A: The gram-positive and negative cells differ in their cell wall composition.
Q: Which bacterial morphology is demonstrated by this simple stain? choose as many as are correct
A: For all types of bacterial cells, a simple staining method may be used to give contrast to the…
Q: Why are bacterial cells generally stained for microscopic viewing?
A: Staining bacterial cells prior to microscopic viewing is a routine process in most of microbiology…
Q: What will happen if you heat fix ypur smear for flagella staining?
A: Falgella staining Flagella is long tail like structure present in most of the bacteria. Flagella…
Q: In gram staining, if bacteria retain the primary stain after decolorizing agent treatment. Then the…
A: Answer: GRAM STAINING = It is the differential staining method to differentiate between gram…
Q: why does sometimes acid-fast bacteria accept the spore stain and spores accept the acid-fast stain.
A: Acid-fast staining technique is a differential staining technique. The bacteria which get stained by…
Q: What external bacterial cell structures can be demonstrated by a negative stain?
A: The negative stain is useful in studying the morphology of bacterial cells. It also characterize…
Q: Why is nigrosin-water- bacteria puddle need to be mixed in a Simple/Negative staining
A: Staining is a technique used in slide preparation to color the specimen. With light microscopy,…
Q: a. Why must heat be used with the application of the primary stain during endospore staining?
A: Defination- An endospore is a non -reproductive structure which is dormant and tough produced by…
Q: List the steps in a Gram stain.
A: Introduction: The principle of Gram's stain depends upon the Gram's reaction. The Gram's reaction…
Q: Staining bacterial capsule can not be accomplished by?
A: Since most standard stains cannot enter the capsule because it is so tightly packed, it is difficult…
Q: When performing a Gram stain on a Gram-negative bacteria, why does the bacteria stain pink? Crystal…
A: Gram staining is a method in which cells stain either pink (gram-negative) or purple (gram-positive)…
Q: What stain is used for mycobacterium?
A: The staining is very important process for observing an organism under microscope. The staining of…
Q: Explain the relationship between bacterial cell wall with gram-positive and gram-negative in the…
A: Gram staining is the standard laboratory practice for distinguishing between two broad species of…
Q: What is the difference between Giemsa and Wright stain?
A: Staining is a technique used to enhance contrast in samples, generally at the microscopic level.…
Q: Why are smears of capsulated bacteria not heat-fixed before staining as opposed to Gram staining?
A: Gram staining is a differential staining technique developed by Danish bateriologist Hans Christian…
Q: What would be the expected outcome of an endospore stain test If the bacteria is a pathogenic…
A: Endospore stain is a differential stain that is used to image endospores in bacteria. A few genera…
Q: List the reagents of the Gram stain technique in order and their general role in the staining…
A: Gram Stain is used to differentiate the gram positive and gram negative bacteria. The gram positive…
Q: Based on the photo, describe the bacteria observed from the different staining technique. -…
A: Microbiology was a blessing in the eradication of mass-destructive epidemics such as rabies,…
Q: In negative staining, the acidic stain will penetrate the cell. In negative staining, it will create…
A: Introduction Staining is a technique for enhancing contrast in material, usually on a microscopic…
Q: move the images of bacterial cells into the chart to illustrate the color of gram-positive and gram…
A: answer given below
Q: if there are features of the gram stain that are not as expected what possible sources or error may…
A: Gram staining is a general technique that is used to differentiate two larger groups of bacteria…
Q: Describe the bacteria observed in different staining procedures. Please refer to the photo - Direct…
A: The cell wall of most bacteria has an overall net negative charge and thus can be stained directly…
Q: what is the function of each one of the Gram-stain reagents?
A: Gram staining is the technique used to classify the bacterial species into broad groups based on the…
Q: Why do we need to stain microorganisms?
A: Microbiology is the study of microbes. Microorganisms include bacteria, fungus, archaea, and…
Q: What is the theory about the mechanism of the Gram-stain reaction? please include…
A: The mechanism of gram staining is employed essentially to differentiate between gram-positive and…
Q: Principle/ technique used in Flagella Stain Method/ Preparation/ Procedure of Flagella Stain
A: Flagella are narrow, slender whip-like structures made by flagellin protein that emerges from the…
Q: Why can't gram staining be used for mycobacterium?
A: Gram staining is one of the staining techniques. This is usually used for differentiating between…
Q: bacteria require staining before microscopic examination?
A: Bacteria are single-celled organisms with a small size. Bacteria can be found practically everywhere…
Q: How does smear preparation of cells from a liquid medium differ from preparation of cells from a…
A: Answer: Smear Preparation is the method in microbiology laboratories to place the small amount of…
Q: It is called a "Negative Stain" because the stain has a negative charge, and is therefore: repelled…
A: An acid dye has negative charge hence are able to bind to the cell structures that are positively…
Q: A staining procedure in which one reagent is used and the bacteria takes up the stain while the…
A: Staining is a technique used to enhance contrast in the sample generally at microscopic level. There…
Q: What is the type of gram stain?
A: Gram stain or Gram staining, also called Gram's method, is a method of staining used to classify…
Q: What color wouod gram negative cells appear if the decolorizing step was left out of staining…
A: Gram staining method: This technique is useful in identifying two groups of bacteria based on the…
Q: For What purpose is Giemsa stain used for?
A: Staining is a technique used in microscopy. Staining is mainly used in biology to highlight the…
Q: Are there any chemical differences between the cell walls of gram-positive and negative bacteria…
A: Bacteria are the prokaryotes which fall under Kingdom Monera. They can be found everywhere. They…
Q: Specific Dyes used in flagella stain: A wet-mount procedure (Ryu method) Dried-smear preparation…
A: Flagella is a hair-like structure that is found in bacteria, archaea, and Eukaryota. It allows the…
Q: List the steps of the gram staining procedure in proper sequence and indicate the color of…
A: Gram staining is a method of staining used to distinguish and classify bacterial species into two…
Q: What staining method would you use to verify that the isolate is not producing survival structures…
A: Staining is the procedure that helps to visualize the minute organisms under a microscope using…
Is the flagellar stain a Positive or Negative Simple Stain protocol?
Trending now
This is a popular solution!
Step by step
Solved in 2 steps
- Why is heat-fixed procedure in bacterial smear preparation not ideal for capsular staining?Describe the Gram staining procedureWhat stain technique is used here? What is the primary stain, mordant, decolorizing agent, and counter stain of this technique? Do we heat fix in here? Describe the organism with purple color. Describe the organism with pinkish red color.
- What stain technique must this be? What is used to stain the background? What is used to stain the bacterial cell (in our method)? What structure is the clear (white) layer? Do we heat fix this? Capsule stain, No heat fixation, crystal violet, copper sulfate,Is the gram stain significant to microorganisms other than bacteria? explainThe primary stain used in the endospore stain is: A. Carbolfuchsin Stain B. Methylene Blue C. Malachite green D. Safranine
- What color wouod gram negative cells appear if the decolorizing step was left out of staining procedure? Why?What makes the Leifson stain different from the other stains used in this exercise? How does it assist in the process of making the flagella visible under the microscope?Additional Differential Staining Procedures: Negative stain, Capsule Stain, Endospore stain, Flagella stain. What is a positive and negative results of this procedures?