Specific Dyes used in flagella stain: A wet-mount procedure (Ryu method) Dried-smear preparation (Leifson staining technique)
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Specific Dyes used in flagella stain:
- A wet-mount procedure (Ryu method)
- Dried-smear preparation (Leifson staining technique)
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- Which of the following serves as a counterstain for the gram-stain technique? It also happens to be the counterstain for the endospore staining technique.Is heat fixation used on bacterial smears that will undergo a capsule stain or flagella stain procedure? Why or why not?Is is best to observe slides of bacteria stained with the acid fast stain under high dry magnification. True or false
- What stain technique must this be? What is used to stain the background? What is used to stain the bacterial cell (in our method)? What structure is the clear (white) layer? Do we heat fix this? Capsule stain, No heat fixation, crystal violet, copper sulfate,Describe the bacteria observed in different staining procedures. Please refer to the photo - Direct Stain - Negative StainExplain in detail gram stain biochemistry, procedures, gram-positive and gram negative cells.
- Additional Differential Staining Procedures: Negative stain, Capsule Stain, Endospore stain, Flagella stain. What is a positive and negative results of this procedures?What stain technique is used here? What is the primary stain, mordant, decolorizing agent, and counter stain of this technique? Do we heat fix in here? Describe the organism with purple color. Describe the organism with pinkish red color.What is the purpose of each reagent in gram-staining? Primary stain Mordant Decolorizing agent Counterstain