Q: What types of samples can be measured with Circular Dichroism? Can information be extracted?
A: Circulate dichroism is the difference in the absorption of left handed circularly polarised…
Q: What is the function of the mordant used in the Gram staining procedure? If the iodine step is…
A: Gram stain or Gram staining, likewise called Gram's strategy, is a technique for staining used to…
Q: What is the principle of freezing of cell lines and primary cells.Describe the general protocol for…
A: Cell culture is a procedure in which cells (either animal or plant cells) are separated from an…
Q: Why is it important to limit the quantity of cells used to prepare a smear?
A: A thin layer of cells or tissues that are taken from the body of an organism under study which is…
Q: n in detail gram stain biochemistry, procedures, gram-positive and gram negative cells.
A: Gram stain, a wide used microbiological, biochemical staining technique that greatly aids in the…
Q: What is the purpose of the pour plate technique? If a pure culture is used to inoculate the plate,…
A: PURPOSE OF POUR PLATE TECHNIQUE The pour plate techniques can be used to determine the number of…
Q: How does pasteurization affect the number of bacteria present in milk? What differences, if any,…
A: To control microbial growth or contamination there are a number of aseptic techniques developed that…
Q: hat is the advantage of culture-independent analysis over culture-dependent analysis of…
A: An unknown microorganism can be identified and classified in a variety of ways. They are divided…
Q: Does the Gram stain provide information regarding a bacterium's susceptibility to antimicrobial…
A: The Gram stain is performed for the determination and classification of the bacteria. In Gram…
Q: In the preparation of a bacterial smear, why is there a need to fix the bacteria to the slide? Aside…
A: Several laboratory techniques necessitate smear preparation. A smear can be made using either broth…
Q: What is the purpose of Thick smear? Purpose of Thin smear?
A: Blood smear:-Also known as peripheral blood smear. -Blood smear has many different uses ranging from…
Q: What is the difference between culture-based technique and culture-independent techniques based on…
A: A microbiological culture, also known as a microbial culture, is a way of multiplying microbial…
Q: What are the disadvantages of doing a total microbial count by examining wet mounts under the…
A: Ans- Wet mounts have the following drawbacks: The benefit of being able to observe movement can also…
Q: Why is it important for a microbiology laboratory technician to use aseptic technique?
A: The majority of the microbes onto which scientists work in laboratories are known pathogens.
Q: Why is it sufficient to stain cells from a SDA (Sabouraud Dextrose Agar) plate with crystal violet…
A: Introduction : Sabouraud agar, often known as Sabouraud dextrose agar (SDA), is a peptone-containing…
Q: Is the flagellar stain a Positive or Negative Simple Stain protocol?
A: Staining is a technique that is used to enhance contrast in samples, generally at the microscopic…
Q: Is heat fixation used on bacterial smears that will undergo a capsule stain or flagella stain…
A: In capsule staining procedure “we do not heat fix and rinse the smear with water” as heat and water…
Q: What are pure cultures and why are they important? How are spread plates, streak plates, and pour…
A: In science and related fields, a culture plate is a low level lined lab holder for growing a layer…
Q: What are the advantages of UV disinfection versus, or as acomplement to, chemical disinfection with…
A: UV disinfection treatment is a very rapid physical process for disinfecting or sterilizing any…
Q: During the preparation of the capsule stain, the slide used to spread the India ink/bacteria mixture…
A: Capsule stain is a type of differential stain which uses acidic and basic dyes to stain the…
Q: Why should culture media after inoculation be incubated at an optimal temperature immediately?
A: Culture media, also called as growth media, are particular combinations of nutrients and other…
Q: Why is it important to use sterile practices when working with microorganisms?
A: Microscopic organisms such as bacteria, fungi (mold and yeast), protists, archaea, algae,…
Q: microbial CELLS is the most RESISTANT to disinfectants and antiseptics?
A: Prions are the infectious agent which are misfolded proteins having the ability to transmit their…
Q: Why is mordant necessary for the staining of bacterial flagella? Answer in not more than 3…
A: In contrary to negative staining, which leaves the bacteria unstained against a dark backdrop,…
Q: What is the secondary stain in both acid-fast stain methods?
A: The bacteria is the unicellular prokaryotic organism that maintains a definite shape since it…
Q: The statement, “In the laboratory, a sterile inoculating loop is moved across the agar surface in a…
A: The technique is streak plate technique. Streak plate method is a pure culture technique which helps…
Q: When using the blue/ white colony screening method, what do blue colonies represent ?
A: Blue- white screening is used for the identification of recombinant bacteria.
Q: What is selective medium?
A: Description of a selective medium.
Q: What should be considered when preparing a monolayer cell culture?
A: Monolayer culture, also known as adherent culture, is a form of animal cell culture in which cells…
Q: is staphylococcus aureus cells under 40 times magnification bigger than onion cell or cheek cell?
A: Staphylococcus aureus is a Gram-positive, round-shaped bacterium that belongs to the Firmicutes and…
Q: Can hand sanitizer kill bacteria?
A: Sanitizer is referred to as any substance or fluid that is used to prevent the spread of pathogens…
Q: Is UV treament more or less effective than disinfection when used against Saphylococcus aureus?
A: Saphylococcus aureus is a gram-positive bacterium that is found in the human nose, skin, and…
Q: How can moist heat sterilization can sterilize antibiotic solution?
A: Moist heat sterilization is a technique that uses heated, increased pressure steam to sterilize an…
Q: What is the procedure for methylene blue stain on e.coli?
A: Methylene Blue is a cationic stain, which binds to negatively charged parts of the cells, such as…
Q: How might a synthetic detergent be superior to a normal soap?
A: INTRODUCTION - Soaps are sodium or potassium fatty acid salts made from hydrolysis of fats in…
Q: What other fields of science besides microbiology is the aseptic technique used?
A: Aseptic technique refers to the procedures that prevent the contamination of microorganisms. They…
Q: How does the agar dilution method differ from streaking toobtain isolated colonies?
A: Both these are methods to culture microorganisms and have their respective pro and cons as mentioned…
Q: what is the function of each one of the Gram-stain reagents?
A: Gram staining is the technique used to classify the bacterial species into broad groups based on the…
Q: Why do we need to stain microorganisms?
A: Microbiology is the study of microbes. Microorganisms include bacteria, fungus, archaea, and…
Q: What is the most difficult type of microbial cell or structure to kill? What methods can be used to…
A:
Q: How do we isolate culture media (step by step process) and what is the purpose of bacterial culture…
A: Introduction :- Microorganisms can be found in the natural environment, such as soil. They coexist…
Q: what is the procedure of solving the probelems of cultures those are suffering severely due to…
A: This is the defense compound of the plant and are toxic in nature because of that preventing plant…
Q: What microbe requires serum components to be added to the growth medium?
A: Introduction Microorganisms:- An organism that can be seen only through a microscope, They live in…
Q: In disc diffusion studies, where will the lowest concentration of the tested chemical be in the agar…
A: The disc diffusion method was developed by Kirby and Bauer to determine the efficiency of a…
Q: If zn stain is positive from a sputum sample, what would be your pre-treatment of choice to isolate…
A: Zn stain test also called as Ziehl-Neelsen test is a rapid method to detect the acid fast bacteria.
Q: Why are plated media stored in an inverted position?
A: Plated media enables the isolation of microorganisms from samples or specimens.
Q: Briefly, in your own words, describe the steps of the gram staining procedure.
A: The bacterial kingdom is classified into Gram positive and Gram negative bacteria depending on their…
Q: Dilute cells from an old culture 1:50 into 200 mL cultures. What volume of the old culture would you…
A: Culture consists of appropriate substances which provides the growth to the desired organisms.
Q: Why did you perform the catalase test on colonies growing on nutrient agar plates but not on the…
A: Catalase test is used to identify the presence of catalase enzyme in the bacteria. Catalase is an…
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- How does smear preparation of cells from a liquid medium differ from preparation of cells from a solid medium? Mark all that apply: A smear made from liquid media will be thinner than a smear made from solid media A smear made from solid media doesn't have to be fixed but a smear from liquid media does A smear made from liquid media will be more thick than a smear made from solid Water must be added to a smear made from solid media but not liquid media A smear made from liquid media doesn't have to be fixed but a smear from solid media does Water must be added to a smear from liquid media but not from solidExplain why only gram-negative cells undergo decolorization during the gram staining procedure. Cite the purpose of each of the following reagents in a differential staining procedure. Primary stain Mordant Decolorizing Agent Counter stain What might happen if the Gram staining procedure is performed on a culture incubated for a little over a dayDilute cells from an old culture 1:50 into 200 mL cultures. What volume of the old culture would you add to the new media?
- Dilute cells from an old culture 1:200 into 5 mL of fresh media. What volume of the old culture would you add to the new media?To dilute a bacterial culture, 500 μl of a 16 hour culture is mixed with fresh culture media to a final volume of 5 ml. How did you calculate it?. Single line text.Why is it necessary to use a 24-hrold culture in gram staining? What does Gram staining reveal owing to its use in clinical diagnosis? Indicate color staining results of gram positive and negative bacteria for every hypothetical scenario. Modification in Procedure Staining results 1. Crystal violet was replaced with methylene blue. G+ G- 2. Mordant was skipped G+ G- 3. Decolorizing step was omitted G+ G- 4. counterstain was not used? G+ G- 5. Crystal violet and safranin was swapped in position. G+ G- What bacterial specimen represent the following: (a) positive simple staining; and (b) negative simple staining viewed under OIO. Provide details such as specimen and bacterial stain used and reference. Positive Staining 1000 X Negative Staining 1000 X
- Put the numbers in the space provided to indicate the order of Capsule Staining. If the statement is not part of the process, put 0. a. Put a loopful of bacteria to the slide. b. Air dry then heat fix the slide. c. Add crystal violet to the slide. d. Add congo red to the slide. e. Apply ethanol.A powdered complex medium can be stored for months in the lab. However, after rehydrating the medium it must be used immediately. Why?As part of an experiment where absorbance values are measured using a spectrophotometer, you are taking readings of your sample every 20 minutes. The non-motile microbe you're testing has a generation time of roughly 20 minutes at an incubation temperature right around room temperature. Things start out fine, with the expected results — as time goes by at the correct incubation temperature, absorbance starts to rise as the medium starts to become more cloudy with growing microorganisms. But roughly 2 hours into the process, you notice that the absorbance levels flatten out, and then start to decrease unexpectedly. What is most likely taking place in your experiment?
- During the preparation of the capsule stain, the slide used to spread the India ink/bacteria mixture is discarded in the disinfectant solution. Why?You have performed an experiment and your aim is to obtain pure colonies. However, you observe some colony variations and you need to verify that this is not due to contamination, name and describe the staining method would you use to achieve this goal?The first step in identifying an organism from a pure working culture is to perform a(n) __________ test. acid-fast staining negative staining endospore staining simple staining Gram stain