Q: What factor allows molecules to be separated by chromatography?
A: Chromatography is a method which is used to separate, identify and purify the components of a…
Q: two chromatograms after LC separation. Which chromatogram is better to proceed for MS analysis? What…
A: LCMS is Liquid chromatography–mass spectrometry used for separation, identification and percentage…
Q: List 2 factors that can affect separation in chromatography experiment
A: 1st factor is by increasing the retardation time. As we increase the size of coloumn ,then analytes…
Q: Please explain how a Flash Column Chromatography works? Please answer at your own words.
A: Introduction : Flask Column Chromatography is a method of chemical separation that is used to…
Q: What is Dry Flash Column Chromatography? Please explain at your own words.
A: Answer - According to the question - Dry Flash Column Chromatography - Dry-column flash…
Q: Which components in a liquid chromatography (LC) instrument are similar to the experimental set-up?
A: As per the guideline, since you have asked multiple questions, we have solved the first…
Q: Discuss various types of paper chromatography?
A: To Find: Explain paper chromatography and it's various types. Paper chromatography is a type of…
Q: What would be the effect of the following errors in chromatographic work? d. The paper or plate is…
A: The Thin layer chromatography works on the principle of separation. There may be some error in…
Q: Why are the components in the sample separated from each other before determination in…
A: Why components of any compound is saparate in chromatography techniques.
Q: chromatography with representative picture.
A: Molecular exclusion chromatography also called size exclusion or gel filtration chromatography . It…
Q: The chromatogram indicates that O MGDG eluted first from the column DGDG SGDG
A: The given chromatogram indicates the retention time of three components.
Q: order to execute paper chromatography, which of the following stages is required? I. Load the…
A:
Q: Explain about Liquid Chromatography technique.
A:
Q: in what situation does one use thin and/or column chromatography? You can any of theme for anything…
A: Chromatography is an analytical technique to separate mixtures into it's components. It is also…
Q: (5). Write short notes on molecular exclusion chromatography with representative picture.
A: Molecular exclusion chromatography, otherwise called Size-exclusion chromatography (SEC), is a…
Q: In chromatography, if the temperature of oven increases, what will be its effect on retention time…
A: Chromatography It is the separation and purification technique used in which a stationary phase and…
Q: What type of components /mixtures can be separated by chromatography
A: Chromatography is a method which usually separate components of a homogeneous mixture based on the…
Q: How do gas-liquid and gas-solid chromatography differ?
A: gas-liquid and gas-solid chromatography difference- Gas - Liquid Chromatography- 1. Gas liquid…
Q: How does paper chromatography allow you to separate substances?
A: Paper chromatography allows you to separate substances has to be determined below.
Q: Why Rf value is important in Chromatography? Please shortly answer at your own words. Answer should…
A: Rf stand for the Retention factor . Its value is equal to the distance travelled by component…
Q: Which compound elutes first and last in reverse chromatography
A: In reverse phase chromatography, stationary phase is non polar and mobile phase is polar in nature.…
Q: What causes the ethanol/isopropyl alcohol to rise up in paper chromatography?
A: A mixture is a type of matter which is composed of two or more substances. These are called…
Q: paper chromatography?
A: Chromatography is a technique that is used for the separation of a mixture. It has two important…
Q: What accounts for a high
A: Chromatography is a technique used for separation and identification of the components in mixture…
Q: Define Flash Column Chromatography? Please answer at your own words
A: The phenomenon of flash column chromatography is related to the chemical analysis method. The word…
Q: What is meant by retention time in gas chromatography. In what order will they come hexane, ethyl…
A: The process of separation of various constituent of a mixture in zone or in phases is known as…
Q: Draw a schematic diagram of how a Flash Column Chromatography works? Please answer at your own…
A: Flash column chromatography: It is a technical chromatography that consumed a compressed gas (such…
Q: What types of flow meters are used in gas chromatography?
A: ANSWER IS
Q: State the major differences along liquid, gas, and super critical fluid chromatography?
A: A supercritical fluid is the phase of a material at critical temperature and critical pressure of…
Q: The flame-ionization detector is used in gas chromatography because the eluates cannot be detected…
A: Detector is the instrument that can detect the eluate species. In gas chromatography analyte…
Q: a pigment in the thin layer chromatography traveled beyond or farther than the solvent front.…
A: As a pigment in the thin layer chromatography traveled beyond or farther the solvent front therefore…
Q: 1. State the reason why we need to compare the observed retention time values to the standard values…
A: As per the rules, only the first question can be answered. Retention time is the time spent by the…
Q: In a normal phase chromatography setup, which would be the predicted characteristics of the eluates…
A: Ans : Eluates will be in decreasing polarity ( Benzoic acid is more polar than benzaldehyde.…
Q: Why does silanization reduce tailing of chromatographic peaks?
A: Given observation, Silanization reduces tailing of chromatographic peaks.
Q: What is the difference between an ascending and descending paper chromatography?
A:
Q: n paper chromatography,
A: Paper chromatography was discovered by Synge and Martin in the year 1943.
Q: Is the mobile phase for your TLC and column chromatography more or less polar than the stationary…
A: Mobile phase: It is the phase that is getting movement in the column. The sample that has to be…
Q: 24. In paper chromatography, a rectangular piece of filter paper serves as the 24. A. mobile phase…
A: Paper chromatography was discovered by Synge and Martin in the year 1943. In paper chromatography,…
Q: What is paper chromatography please include the importance of using it
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Q: Explain why the indicators, red and blue, are separated on the TLC plate during a chromatography…
A: Thin ayer chromatography is a chromatography technique, which is used to separate non-volatile…
Q: Separation of Peaks Below are two chromatograms after LC separation. Which chromatogram is better to…
A: LCMS is Liquid chromatography–mass spectrometry which is used for mass analysis of unknown or known…
Q: What is Jon chromatographys
A: Given To know about the chromatography
Q: In thin layer chromatography, the stationary phase is made of and the mobile phase is made of solid,…
A: Thin layer chromatography: TLC is a chromatography technique used to seperate non-volatile mixture.…
Q: "Start the chromatography by adjusting the fraction collector to 130 drops per fraction (about 3…
A: It is given that for 130 drops per fraction it shows volume 3 mL That means volume of 130 drops is 3…
Q: is the chromatography in reverse or normal phase if the standards and the sample are spotted in a…
A: The given type of paper chromatography is normal phase or reverse phase explanation is given below…
Q: Sketch a chromatogram for a compound with a stronger interaction with the stationary phase than the…
A:
Q: In column chromatography, a) what constitutes the stationary phase, and b) what is the mobile phase…
A: By definition, chromatography is a separation technique in which a sample is equilibrated between a…
Q: Differential migration of the components is called a of the chromatogram.
A: Differential migration is the basis of chromatography. In a mobile phase, the solutes transit…
Q: Comments on choice of a paper and solvent are main back bones of paper chromatography.
A: Paper Chromatography is mailny based on the choice of paper and solvent used. In this technique,…
Is the paper chromatography used normal or reverse?. Explain why
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- Louie performed paper chromatography to identify which among the four availablereference mixtures — WAR, OFF, YIN, MEW — have the same components as hisunknown sample, GUN. He spotted the standards and the sample in a Whatman No. 1filter paper, which he placed in a developing chamber containing 3% KCl. After 20minutes, Louie obtained the chromatogram below, immediately noting that the distancetravelled by the solvent from the origin is 79.0 mm. On the other hand, the distances (incm) traveled by the developed spots were tabulated as follows.(see attached) Questions: 1. Is the type of paper chromatography performed normal or reversed? Why?2. Calculate the Rf of the spots from the samples. Report your answers in four decimal places.A scientist wishes to measure the concentration of methyl benzoate in a plant stream by gas chromatography. He prepares a sample of butyl benzoate to use as an internal standard. The results of a preliminary run, which used a solution known to contain 1.37 mg/mL of methyl benzoate (peak A) and 1.51 mg/mL of butyl benzoate (peak B), are shown. The area of peak A is determined to be 3.40×102 and the area of peak B is determined to be 398 measured in arbitrary units by the computer. To measure the sample, 1.00 mL of a standard sample of butyl benzoate containing 2.49 mg/mL is mixed with 1.00 mL of the plant stream material. Analysis of the mixture gave a peak area of 437 for peak A and 415 for peak B. What is the concentration of methyl benzoate in the plant stream?In ion-exclusion chromatography, ions are separated from nonelectrolytes (uncharged molecules) by an ion-exchange column. Nonelectrolytes penetrate the stationary phase, whereas ions with charge of the same sign as that of the stationary phase are repelled by the stationary phase. Because electrolytes have access to less of the column volume, they are eluted before nonelectrolytes. A mixture of trichloroacetic acid (TCA, pKa 5 20.5), dichloroacetic acid (DCA, pKa 5 1.1), and monochloroacetic acid (MCA, pKa 5 2.86) was separated by passage through a cation-exchange resin eluted with 0.01 M HCl. The order of elution was TCA , DCA , MCA. Explain why the three acids are separated and the order of elution.
- Earth, a CHEM 40.1 student, extracted a mixture of pigments from the roots of Rubiatinctorum, commonly known as rose madder. Earth spotted the root extract on twohydrophobic silica gel plates. He then placed each plate in separate developing chambers,one containing solvent system WHITE and the other housing solvent system MIX. Earthallowed the chromatograms to develop for around five minutes. Afterwards, he removedthe plates from the containers and placed them inside an iodine chamber. Thechromatograms resulting from the TLC experiment after visualization are illustrated asfollows. The distances travelled by each spot and by the solvents were measured andrecorded in the displayed table.Use the following information collected from a SIZE-EXCLUSION CHROMATOGRAPHY experiment to answer the questions below. MW Standards Molecular Weight (kDa) Elution Volume (mL) blue dextran 1000 85 myoglobin 17.2 200 TIM 53.3 178 hemoglobin 62.2 156 IgG 140 122 ATCase 307.9 94 Prepare a graph showing the linear relationship between log function of molecular weight against Ve/Vo. The R2value must be 0.9 or higher. What is the linear equation from the standards given? What is the R2value?Three students did a chromatography experiment, where Rf = distance of solute / distance of solvent. What could be the possible errors why student 3 had results that are quite far from that of students A and B?
- Arrange in INCREASING Rf if the mixture underwent paper chromatography with the same solvent system. Explain how you get the answer I. 2-chloropentaneII. pentanoic acidIII. cyclopentaneIV. pentanal a. II = IV < III < I b.II < IV < I < III c.I < II < III < IV d.III < I < IV < IISuppose that you try separating an unknown mixture by normal-phase chromatography with the solvent mixture 50% hexane-50% methyl t-butyl ether (which is more polar than hexane). The peaks are too close together and are eluted rapidly. Should you use a higher or lower percentage of hexane in the next run?Consider the following statements as they apply to gas chromatography. Decide if each is true or false. 1. ___ Temperature programming is necessary only for samples containing compounds with a narrow range of boiling points. 2. ___ GC carrier gases are generally inert gases like helium and nitrogen. 3. ___ The injection port temperature should be set at the average boiling point of all analytes. 4. ___ An FID is a mass-sensitive detector, not a concentration-sensitive device. 5. ___ An air peak is commonly used to determine the dead time when using an FID detector.
- 1. For the following chromatography state what the mobile phase is, stationary phase is, and what would elute first for each of the chromatography type... HPLC Gas Chromatography Ion Exchange Chromatography Size Exclusion Chromatography (SEC) Affinity Chromatography Reverse Phase Chromatography Normal Phase ChromatographyIman has a mixture of two compounds A and B. A is a protein, and B is a lipid. He plans to separate the mixture using a column chromatography technique. He packed the column with amino bonded with siloxane material and use a mixture of 20:80 of water: hexane as the mobile phase. i) Sketch the apparatus set up for the chromatographic technique used by Iman. Use the correct chromatographic terminologies to label all of the apparatus, materials, and phases. ii) Predict the sequence of elution when the mixture was loaded onto the separation column. Justify your answer. iii) After the first compound was eluted, Iman discovered that the second compound was taking too long to pass through the column. Please suggest any strategies that could be used to aid the elution process.Qno1: why there is a need of chromatography and chromatography thecniqs. B) when and where we should use normal and reverse phase chromatography
