* Question Completion Status:11121314151617181920212223242834353637383940414243444546474849505253545556575859606140어 ADGDGMGDGSQDGmV 200-0-1015 minThe chromatogram indicates thatO MGDGeluted first from the columnDGDGSGDGQUESTION 53Click Save and Submit to save and submit. Click Save AIL Answers to save all answers.Save All A

The given chromatogram indicates the retention time of three components.

Answered: The chromatogram indicates that O MGDG…

Science

Chemistry

The chromatogram indicates that O MGDG eluted first from the column DGDG SGDG

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter4: Digital Electronics And Computers

Section: Chapter Questions

Problem 4.11QAP

See similar textbooks

Related questions

Q: What factor allows molecules to be separated by chromatography?

A: Chromatography is a method which is used to separate, identify and purify the components of a…

Q: Prelaboratory Assignment for Paper Chromatography 0 The 1 SAYIY

A: Note : Compound A is more soluble in mobile phase. Therefore compound A is less polar and having…

Q: two chromatograms after LC separation. Which chromatogram is better to proceed for MS analysis? What…

A: LCMS is Liquid chromatography–mass spectrometry used for separation, identification and percentage…

Q: How chromatogram is developed in thin layer chromatography?

A: Chromatography is a technique used to separate mixture of substances into their components. It…

Q: Retention Time Area of Peak % of Total Identity Component 1 Component 2 Component 3

A: Given data:

Q: Why is it necessary to cover the developing chamber tightly during the development of a…

A: Given : Development of chromatogram To find: Reason for tightly packed developing chamber during…

Q: A spot of black ink was placed on a strip of filter paper. The chromatogram was developed using an…

A: The fastest moving spot has the highest Rf value. here we see C covered a long distance so it has…

Q: Using the chromatogram shown here, which was obtained on a 2-m column, determine values for tr, w,…

Q: A chromatogram from a certain column has a peak with a w1/ 2 of 12 mm and a tr of 650 mm as…

A: Given, Width of half peak height , w12= 12 mm retention time , tr = 650 mm

Q: Why are the components in the sample separated from each other before determination in…

A: Why components of any compound is saparate in chromatography techniques.

Q: The portions of the sample having the analyte with similar composition analyzed using the same…

A: The portions of the sample having the analyte with similar composition analyzed using the same…

Q: a. What does ECD mean. b. Discuss how column temperature influences separation in a chromatography…

A: “Since you have posted a question with multiple sub-parts, we will solve the first three subparts…

Q: Red, blue, and yellow spots were found in the chromatogram. Red spot traveled twice the distance…

Q: How many theoretical plates produce a chromatography peak eluting at 12.83 min with a width at…

A: The no. of theoretical plates can be calculated as: Where N is the no. of theoretical plates, tr is…

Q: In chromatography, if the temperature of oven increases, what will be its effect on retention time…

A: Chromatography It is the separation and purification technique used in which a stationary phase and…

Q: What type of components /mixtures can be separated by chromatography

A: Chromatography is a method which usually separate components of a homogeneous mixture based on the…

Q: How can I calculate the concentration from a GC/MS chromatogram which is having retention time and…

A: GC-MS chromatography also called the gas chromatography mass spectrometry is an analytical tool to…

Q: Which of the following statements is incorrect? O A. In reversed-phase chromatography, the…

Q: calculate the following for each sample gas chromatograph. a) retention time for each peak (label…

A: The given plot is redrawn as shown below.

Q: How does paper chromatography allow you to separate substances?

A: Paper chromatography allows you to separate substances has to be determined below.

Q: a. Calculate the retention factor for each spot from lanes A to E . b. Which ink standard was the…

A: Paper Chromatography is a technique where paper is used as the stationary phase through which the…

Q: Consider the peaks for heptane (14.56 min) and p-difluorobenzene (14.77 min) in the chromatogram.…

A: a.

Q: what do the different colors in the result of paper chromatography experiment indicates? 2. Why do…

A: Paper chromatography is a technique in which colour chemicals or substances are separated.

Q: Analysis of an unknown plant extract yield two peaks X and Y in a chromatogram, 15.65 and 17.03 min…

Q: Why Rf value is important in Chromatography? Please shortly answer at your own words. Answer should…

A: Rf stand for the Retention factor . Its value is equal to the distance travelled by component…

Q: Analysis of an unknown plant extract yield two peaks X and Y in a chromatogram, 15.65 and 17.03 min…

Q: The plot of retention time versus detector signal in chromatography called?

A: In the GC-plot, the detector response signal is at the y-axis, and the retention time is at the…

Q: paper chromatography?

A: Chromatography is a technique that is used for the separation of a mixture. It has two important…

Q: calculate the following for each sample gas chromatograph. a) retention time for each peak (label…

A: Gas chromatography is a technique for separating and analyzing compounds that can be vaporized…

Q: Analysis of an unknown plant extract yield two peaks X and Y in a chromatogram, 15.65 and 17.03 min…

A: Two peaks X and Y in a chromatogram yields in 15.65 and 17.03 min respectively in a 30 cm column.…

Q: The flame-ionization detector is used in gas chromatography because the eluates cannot be detected…

A: Detector is the instrument that can detect the eluate species. In gas chromatography analyte…

Q: a pigment in the thin layer chromatography traveled beyond or farther than the solvent front.…

A: As a pigment in the thin layer chromatography traveled beyond or farther the solvent front therefore…

Q: would column chromatography separate ketone and alcohol

A: The detailed solution of the given question is solved below. COLUMN CHROMATOGRAPHY Column…

Q: Given the chromatogram shown below, answer the following questions: (a) Match the following…

A: In reverse phase chromatography polar solvent is used as mobile phase. As a result of which polar…

Q: Column A Cesponse Unretained peaks Compound Y Compound X Time (min) 8 min. Compound Y Column B…

A: We can decide the effectiveness of resolution in column chromatography based on the nature of the…

Q: Separation of Peaks Below are two chromatograms after LC separation. Which chromatogram is better to…

A: LCMS is Liquid chromatography–mass spectrometry which is used for mass analysis of unknown or known…

Q: True or False: Thin-layer chromatography yields thepositive identification of a material.…

A: Thin Layer chromatography in one of the important chromatographic technique which is most widely…

Q: What is Jon chromatographys

A: Given To know about the chromatography

Q: calculate Rfs for each spot on your chromatogram

A: Retention factor is equal to the ratio of distance travelled by solute divided by the distance…

Q: In a chromatographic experiment, a mix of 3 compounds are separated. The following data are…

A: The mass percentage of compound C is needed to be calculated from the given chromatographic data.

Q: Name two general methods for improving the resolution of two substances chromatographic column.

A: Good resolution columns provide a great separation of the components by a column chromatography.…

Q: In thin layer chromatography, changing the mobile phase will alter the Rf value of a compound. O…

A: This can be solved as follows

Q: An ion chromatograph is used for the determination of chloride ions. Seven replicate asurements of…

A: Given: Number of replicates = 7 The replicate measurements obtained are 20, 21, 18, 22, 20, 22, and…

Q: How we optimize chromatographic separations?

A: Chromatographic separation of mixture of various compounds are based on their distribution between a…

Q: Determination of ascorbic acid in vitamin C tablets: which analytical approach is better; volumetric…

A: Determination of ascorbic acid from different species of citrus fruits. UV Spectrophotometry is…

Q: For any chromatographic technique, tall and narrow peaks are preferred to short and broad peaks. O…

Q: How is the signal intensity seen in the chromatogram affected by the wavelength used for analysis?

A: Chromatography is a group of methods for separating very small quantities of complex mixtures, with…

Q: what is the effect on the chromatographic peak if a. inserting/adding sample too slow b. column…

A: Effect of chromatographic peak on a. inserting/adding sample too slow b. column temperature is too…

Q: Gas chromatograph is usėd to measure 1) CO and CO2 3) CO and NO 2) CO and UBHC 4) CO and NO2

A: 1) CO and CO2

Question

Expert Solution

This question has been solved!

Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.

This is a popular solution!

SEE SOLUTION Check out a sample Q&A here

Step 1

VIEW

Step 2

VIEW

Trending now

This is a popular solution!

Step by step

Solved in 2 steps

SEE SOLUTION Check out a sample Q&A here

Knowledge Booster

Learn more about

Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, chemistry and related others by exploring similar questions and additional content below.

Similar questions

Use the following information collected from a SIZE-EXCLUSION CHROMATOGRAPHY experiment to answer the questions below. MW Standards Molecular Weight (kDa) Elution Volume (mL) blue dextran 1000 85 myoglobin 17.2 200 TIM 53.3 178 hemoglobin 62.2 156 IgG 140 122 ATCase 307.9 94 Prepare a graph showing the linear relationship between log function of molecular weight against Ve/Vo. The R2value must be 0.9 or higher. What is the linear equation from the standards given? What is the R2value?
I'm not sure how to approach this problem because the ruler on the chromatogram starts at 2. I need help finding the distance for the 3 species traveled.
In a chromatographic analysis, it was found out that distance travelled by the alcohol solvent is 4 cm away from the finish line. The distance travelled from the starting line to finish line is 40 cm. The first amino acid, AA1 travelled 6 cm behind the distance travelled by the alcohol. The second amino acid, AA2 travelled 12 cm short from the solvent front while the third amino acid, AA3 travelled 8 cm ahead of AA2. Calculate: Rf of AA1 ____ Rf of AA2____ Rf of AA3____ (Rf= Retention factor)
1. For the following chromatography state what the mobile phase is, stationary phase is, and what would elute first for each of the chromatography type... HPLC Gas Chromatography Ion Exchange Chromatography Size Exclusion Chromatography (SEC) Affinity Chromatography Reverse Phase Chromatography Normal Phase Chromatography
1) a)What is affinity chromatography and what is it used for? b) A drug called Bamlanivimab is used to treat infections with SARS-CoV-2 (Covid-19).This drug was developed by isolating antibodies from the serum of a patient who recovered from Covid-19.What is possibly on the stationary phase in a column used to isolate antibodies to SARS-CoV-2? How are the antibodies eluted off the column to isolate them for drug development?
You run the chromatography system with paper as the stationary phase and 70% isopropanol as the mobile phase. You determine the Rf of a polar analyte to be 0.40. If you use 100% isopropanol (a less polar solvent) instead of isopropanol as the mobile phase what would you expect to happen to the Rf value? No value effect on Rf higher lower could be higher or lower
In a normal phase chromatography setup, which would be the predicted characteristics of the eluates based on retention time? eluates will be in decreasing polarity eluates will only contain the mobile phase eluates will only contain the stationary phase eluates will be in increasing polarity
Determine the concentration of linalool in a highly valued lavender essential oil. The peak areas of a series of standard solutions (calibrations standards) acquired from GC-MS (gas chromatography-mass spectrometry) is presented below. Concentration (mg/L) Peak area 0 201 25 5214 50 14658 75 23647 100 32657 125 42652 150 52478 QUESTION: Calculate the concentration of linalool in yourhighly valued lavender essential oil which returns a peak area of 27235.
q41 IF YOU DO NOT KNOW THE ANSWER PLEASE DO NOT REJECT IT please calculate the unknown concentration of the protein D wih an absorbance value of A412 given the standard curve indicated in the table. write your answers in numbers only with 2 decimals. protein concentration (ug/ml) absorbance 0 0.000 0.02 0.161 0.04 0.284 0.06 0.438 0.08 0.572 0.10 0.762
In which mode of chromatography do solute and solvent molecules migrate in and out the stationary phase based on the solute’s equilibrium between the stationary and mobile phases. a) adsorption b) partition c) extraction d) absorbance
You finish doing an experiemnt with Benzoin. These are the results you get:Boiling start point: 137 ceciusBoiling end point: 140 ceciusVile while empty (with cap on): 15.348Vile with crude prod (with cap on: 15.748 Your starting weight: 1.00115.748 - 15.348 = 0.400 / 1.001 = 0.3996 or 39.96% YieldAnswer the following questions with this information:Initial Mass of impure sample:Mass of recrystallized benzoin:Percent reccovery of Benzoin:Melting pot range of purified benzoin:Literature melting point for benzoin:
avg concentration: 8.937x10^-7