Answered: It is time to transfect the construct…

It is time to transfect the construct of your choice into mouse cells. What type of mouse cells should be transfected? What is the rationale to use that particular cell type?

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter17: Genes And The Immune System

Section: Chapter Questions

Problem 13QP: The Adaptive Immune Response Is a Specific Defense Against Infection In cystic fibrosis gene...

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Concept explainers

Clinical Pharmacology

Pharmacology is a discipline of medicine that studies how medications interact with the systems and processes of living organisms, focusing on drug action mechanisms as well as therapeutic and other purposes.

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It is time to transfect the construct of your
choice into mouse cells. What type of
mouse cells should be transfected? What is
the rationale to use that particular cell type?

Expert Solution

Step 1

Downstream analysis of gene constructs in mammalian system is essential to understand their effects at genetic level. Embryonic cells have an advantage over other cell types due to their indefinite proliferation and renewal and its capacity to differentiate into all germ lines, namely ectoderm, mesoderm and endoderm. Moreover, these cells can easily be modified genetically using a variety of techniques like liposomes, chemicals, viruses and electroporation.

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