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- If 5 µM enzyme was used to obtain the data in the summary plot below, V-max = ________________ µM sec-1 and Km = ____________ µM for this enzyme (Enter numeric values to the nearest integer; Do NOT write units.)Q) water hardness of each trial and average ppm with calculation, please. Hard Water Trial 1 Hard Water Trial 2 Hard Water Trial 3 Initial Syringe Reading 1.0ml 1.0ml 1.0ml Final Syringe Reading 0.88ml 0.84ml 0.85ml Volume of EDTA Consumed 0.12ml 0.16ml 0.15ml Water Hardness ppm CaCO3 Average ppmWhich statements are TRUE regarding internal standards? I. The response factor is calculated by using a mixture containing known amounts of analyte and internal standard and by using detector response to the analyte and the internal standard. II. Internal standards are useful where instrument response or quantity of sample analyzed varies over time. III. Detector response is inversely proportional to concentration. IV. Detector response to analyte and internal standard is assumed to be constant over a range of concentrations A. I, II, and III B. II, III, and IV C. I and II D. I, II, and IV
- please help this is my 4th time posting this and i havent gotten a correct answer. Please actually draw the graph out. A newly discovered enzyme, MBCase, cleaves carbon-carbon bonds of vicinal alcohol; The kinetic parameters were measured for 1.0 nM Of MBGase and determined to be a Km = 10 nM and a kcat = 100 s-1 a.) Sketch the graph out as a double-reciprocal plot (Lineweaver-Burke Plot) and label: -the axes (with units) -the point on the curve that can be used to determine Km -the point on the curve that can be used to determine Vmaxprepare an assignment on Application of HPLC in Drug Analysis(Amiloride): one page show name of the drug, dosage form, extraction step, type of column, separation parameters e.g., flow rate, mobile phase, detection wavelength and run timeA fly ash aerosol of monodisperse particles with a density of 2.0 g/mL and particle diameter of 10.0 mm has a concentration of 1000.0 mg/m3. Calculate the settling velocity in cm/s and the settling rate in g/m2-s. Please answer very soon will give rating surely
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