Km Value Vmax Value kcat Value

Principles of Instrumental Analysis
7th Edition
ISBN:9781305577213
Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Chapter30: Capillary Electrophoresis, Electrochromatography, And Field-flow Fractionation
Section: Chapter Questions
Problem 30.6QAP
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V3

Using the information provided below, calculate the Km and Vmax values for this kinetics trial.
Also, determine the kcat value for [E] = 0.05µM. Pay attention to Sig Figs and to Units.
Km Value
Vmax Value
kcat Value
0.4
y = 0.6882x + 0.0736
V
1/S
1/V
0.3
mM
mM/min
1/mM
min/mM
3.66
0.333
0.295
-0.2
5.12
0.200
0.230
7
6.18
0.143
0.173
6.98
0.111
0.143
11
7.61
0.091
0.131
-0.2 -0.1
0.1
0.2 0.3 0.4
1/S (1/mM)
Transcribed Image Text:Using the information provided below, calculate the Km and Vmax values for this kinetics trial. Also, determine the kcat value for [E] = 0.05µM. Pay attention to Sig Figs and to Units. Km Value Vmax Value kcat Value 0.4 y = 0.6882x + 0.0736 V 1/S 1/V 0.3 mM mM/min 1/mM min/mM 3.66 0.333 0.295 -0.2 5.12 0.200 0.230 7 6.18 0.143 0.173 6.98 0.111 0.143 11 7.61 0.091 0.131 -0.2 -0.1 0.1 0.2 0.3 0.4 1/S (1/mM)
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