Answered: Reaction velocity (µM · s-1) Substrate…

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Reaction velocity (µM · s-1) Substrate concentration (µM) 1 5 100 50

Fundamentals Of Analytical Chemistry

9th Edition

ISBN:9781285640686

Author:Skoog

Publisher:Skoog

Chapter30: Kinetic Methods Of Analysis

Section: Chapter Questions

Problem 30.9QAP

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Question

You are trying to determine the K_M for an enzyme. Due to a lab mishap, you have only two usable data points: Use these data to calculate an approximate value for K_M. Is this value likely to be an overestimate or an underestimate of the true value? Explain.

Reaction velocity
(µM · s-1)
Substrate
concentration (µM)
1
5
100
50

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How did you get 0.32?

Also where did you get 500 for Vmax? In the question it says 50

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