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- Both microarray and RNA-sequencing can study the transcriptomes. Compare microarray and RNA sequencing technique in analyzing gene with high, medium and low copy number of genes.Describe how Ion torren method of sequencing works in detail.Briefly explain how synthetic probes are created to screen a DNA library when the protein encoded by the gene is known.
- What advantages do cDNA libraries provide over genomic DNA libraries? Describe cloning applications where the use of a genomic library is necessary to provide information that a cDNA library cannot.Discuss the underlying biochemical principle of the nucleic acid sequencing methods known as Semiconductor (Ion Torrent) sequencing.Explain why the 16S rRNA gene sequencing is suitable for bacterial identification in general and why it is mostly limited to genus level.
- Refer to the figure. What method would you use if you wanted to determine the sequence of the cDNA of the peak protein? Briefly explain the sequencing methodWhat is dideoxy sequencing? Explain it please.Please list all possible products in a sequencing reaction using ddGTP as a terminator based on the following sequence of a template. 3’ – GGGCTATACCTGACCCTC – 5’