LO73 Determine which nucleotides are methylated based on a sequence that has been treated with sodium bisulfite. Which of the following is NOT true of bisulfite sequencing? All thymines revealed in DNA sequencing of bisulfite treated DNA were cytosines in the original, untreated DNA. ODNA is treated with sodium bisulfite, which chemically converts unmethylated cytosines to uracil. The sequence of genomic DNA treated with sodium bisulfite is compared to the sequence of untreated genomic DNA. Unmethylated cytosines are detected as thymine sequencing. Methylated cytosines are unaffected by sodium bisulfite. .

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
icon
Related questions
icon
Concept explainers
Question
LO73 Determine which nucleotides are methylated based on a sequence that has been treated with sodium
bisulfite.
Which of the following is NOT true of bisulfite sequencing?
All thymines revealed in DNA sequencing of bisulfite treated DNA were cytosines in the original, untreated DNA.
ODNA is treated with sodium bisulfite, which chemically converts unmethylated cytosines to uracil.
The sequence of genomic DNA treated with sodium bisulfite is compared to the sequence of untreated genomic
DNA.
Unmethylated cytosines are detected as
duri sequencing.
Methylated cytosines are unaffected by sodium bisulfite.
→
Transcribed Image Text:LO73 Determine which nucleotides are methylated based on a sequence that has been treated with sodium bisulfite. Which of the following is NOT true of bisulfite sequencing? All thymines revealed in DNA sequencing of bisulfite treated DNA were cytosines in the original, untreated DNA. ODNA is treated with sodium bisulfite, which chemically converts unmethylated cytosines to uracil. The sequence of genomic DNA treated with sodium bisulfite is compared to the sequence of untreated genomic DNA. Unmethylated cytosines are detected as duri sequencing. Methylated cytosines are unaffected by sodium bisulfite. →
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 2 steps

Blurred answer
Knowledge Booster
DNA and RNA
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education