- [methyl-1“C]Pyruvate was administered to isolated liver cells in the presence of sufficient malonate to block succinate dehydrogenase completely. After a time, isocitrate was isolated and found to contain label in both carbon 2 and carbon 5: 14CH,-Coo HC-COo H14C-coo OH How do you explain this result?
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![- [methyl-1“C]Pyruvate was administered to isolated liver cells in the
presence of sufficient malonate to block succinate dehydrogenase
completely. After a time, isocitrate was isolated and found to contain
label in both carbon 2 and carbon 5:
14CH,-Coo
HC-COo
H14C-coo
OH
How do you explain this result?](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fcc6bfc5e-a79d-4d57-905c-7fb9a6e63596%2F1c6bb173-5175-4f86-b964-8ad834d46239%2F6wbw7do_processed.png&w=3840&q=75)
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- [methyl-14C]Pyruvate was administered to isolated liver cells in the presence of sufficient malonate to block succinate dehydrogenase completely. After a time, isocitrate was isolated and found to contain label in both carbon 2 and carbon 5: How do you explain this result?The isoelectric point (pI) of 6-phosphogluconate dehydrogenase is 6.0. Explain why the buffer used in DEAE cellulose chromatography must have a pH greater than 6 but less than 9 in order for the enzyme to bind to the DEAE resin.In some brain cancer cells, a mutated form of isocitrate dehydrogenase, instead of catalyzing the oxidation of the secondary alcohol of isocitrate, catalyzes the reduction of a-ketoglutarate. Draw the product of the reaction.
- Some diazotrophs produce a vanadium-containing VFe protein in addition to the MoFe protein. The vanadium-containing nitrogenase converts N2 to NH3 and also converts CO to compounds such as ethane and propane. What aspect of the standard nitrogenase reaction is responsible for the production of alkanes?If 14CO2 (radioactive carbon) were incorporated into the TCA cycle via the Pyruvate Carboxylase reaction which of the following molecules would contain radioactive carbon? a. Oxaloacetate b. Citric Acid c. Isocitrate d. Alpha-ketoglutarate e. Succinyl-CoA f .Succinate g. Fumarate h. MalateWith alanine AA, and using citric acid cycee and glycolysis, which Carbon atom would be labeled 1st with 14C n succinate? Why?
- a-Keto acids other than a-ketoglutarate can accept the amino group from pyridoxamine in enzyme-catalyzed transamination reactions. What amino acids are formed when the following a-keto acids accept the amino group?O OO O Opyruvate oxaloacetateAlcohol dehydrogenase catalyzes the oxidation of an alcohol to the next highest state of oxidation, an aldehyde. How does this partially explain the toxicity of methanol?Refer to Figure, which indicates ΔG for each glycolytic reaction under intracellular conditions. Assume that glyceraldehyde-3-phosphate dehydrogenase was inhibited with iodoacetate, which reacts with its active site cysteine sulfhydryl group. Which glycolytic intermediate would you expect to accumulate most rapidly, and why?
- If aspartate is labeled with deuterium D (= H-2 = 12H), answer questions a-c to determine where the label will end up in oxaloacetate after the involvement of aspartate in the urea cycle. Where does the label end up in arginosuccinate? Where is the label in fumarate? Where is the label in oxaloacetate?Which of the following glycosidic linkages is hydrolyzed by the α-amylase? α(1,6) α(1,4) β(1,4) α(1,2)Why is the glyoxylate cycle necessary for growth on acetate butnot on succinate?