what practical importance are air borne microorganisms to the laboratory workers?  What precautions should be taken to control laboratory contaminants?  What precautions should be taken to control laboratory contaminants? Of what advantage is the using a solid and a liquid medium

Principles Of Pharmacology Med Assist
6th Edition
ISBN:9781337512442
Author:RICE
Publisher:RICE
Chapter15: Antibiotic Agents
Section: Chapter Questions
Problem 20RQ
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Of what practical importance are air borne microorganisms to the laboratory workers? 

What precautions should be taken to control laboratory contaminants? 

What precautions should be taken to control laboratory contaminants?

Of what advantage is the using a solid and a liquid medium?  

MICROORGANISMS IN THE ENVIRONMENT
II. Materials
O 7 sterile petri dishes
Sterile nutrient agar
O Water bath
III. Procedures:
1. Melt nutrient agar in a water bath and cool to approximately 45-degree Celcius.
2. Into each sterile petri dish, pour about 15-20 ml nutrient agar following aseptic technique.
3. Upon solidification of the medium, label dishes A, B, C, D, E, F, & G.
A. Remove cover and swab a tv remote control then rub it over the surface of the agar,then cover the
plate.
B. Remove cover and cough directly into the agar surface 2 to 3 times then cover the plate.
C. Remove cover and rub tips of your fingers over the surface of the agar then cover the plate.
D. Remove cover and swab the sole of the shoe and rub tips over the surface of the agar, then cover the
plate.
E. Pick up a small amount of dust on a piece of cotton and rub over the agar surface, cover the plate.
F. Open the petri dish and pass it over the hair, then cover.
G. Leave this plate untouched.
4. Incubate the plates in an inverted position at 37-degree Celcius. Make your observation after three
days.
Transcribed Image Text:MICROORGANISMS IN THE ENVIRONMENT II. Materials O 7 sterile petri dishes Sterile nutrient agar O Water bath III. Procedures: 1. Melt nutrient agar in a water bath and cool to approximately 45-degree Celcius. 2. Into each sterile petri dish, pour about 15-20 ml nutrient agar following aseptic technique. 3. Upon solidification of the medium, label dishes A, B, C, D, E, F, & G. A. Remove cover and swab a tv remote control then rub it over the surface of the agar,then cover the plate. B. Remove cover and cough directly into the agar surface 2 to 3 times then cover the plate. C. Remove cover and rub tips of your fingers over the surface of the agar then cover the plate. D. Remove cover and swab the sole of the shoe and rub tips over the surface of the agar, then cover the plate. E. Pick up a small amount of dust on a piece of cotton and rub over the agar surface, cover the plate. F. Open the petri dish and pass it over the hair, then cover. G. Leave this plate untouched. 4. Incubate the plates in an inverted position at 37-degree Celcius. Make your observation after three days.
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