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- Describe the intracellular, biochemical downstream signal cascade that results from the binding of menthol to TRPM8Explain how the pKa for histidine 57 is important to its role in the mechanism of chymotrypsin actionexplain the following prperties of G protein: structure of G- activation cycle and signaling pathway for Gaq
- Activation of phosphatidylinositol-3-kinase (PI3K) stimulate protein-protein interactions. True or FalseDescribe the importance of ubiquitin-dependent degradation of soluble proteins.(Note: The alpha subunit of the heterotrimeric G-protein complex becomes activated upon activation of a GPCR. ) What is the mechanism used to turn off the alpha subunit ?
- Describe the similarities and differences in the structures of GPCRs specific for various ligands including the extracellular , transmembrane , and intracellular domains.Ion channel-coupled receptors • G-protein-coupled receptors • Enzyme-linked receptors • Briefly describe what a G-protein is (explain by using the words GTPase, GTP and GDT) • Briefly describe how a G-protein-coupled receptor for the signal / information further into • Briefly describe how an enzyme-linked receptor carries the signal / information further into the cell.Explain how can cAMP’s activation of a single molecule, cAMPdependent protein kinase, be common to the great variety of biochemical sequences and cell responses initiated by cAMP-generating first messengers?
- Draw a fractional binding curve for trimeric protien that has a binding site on each subunit. Moreover, the protein has a preferred low affinity conformation.CK1-41 is a non-competitive inhibitor of caspase-1. Explain.A study is conducted to assess the molecular mechanisms of hormone X. A radioactively labeled form of this hormone is incubated with human cells in tissue culture for 30 minutes, and then incubated briefly with a cross-linking agent. The plasma membrane, cytosolic, and nuclear fractions are isolated from the cell lysate, and the subcellular proteins are separated from these fractions by gel electrophoresis. The only protein bound to the labeled hormone is identified in the new clear fraction. Hormone X is most likely which of the following? Epinephrine Glucagon Growth hormone Insulin Thyroxine