1. In the SDS-PAGE figure below, looking at lane 6 there are two bands MamP,monomer and MamP dimer (disulfide bonded).Why does the MamP monomer run faster on the gel relative to the MamP dimer?1 2 3 4 5 6250 -130 -1007055- Hisg MBPtev.MamfMamP dimer35 -25-- MamP monomer15 -10 -If the gel was run under non-reducing conditions, would the pattern possibly bedifferent and why?Molecular weight (kD)

A dimer is an oligomer consisting of two monomers joined by bonds that can be either strong or weak…

Answered: monomer and MamP dimer (disulfide…

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monomer and MamP dimer (disulfide bonded). Why does the MamP monomer run faster on the gel relative to the MamP dimer?

Biology 2e

2nd Edition

ISBN:9781947172517

Author:Matthew Douglas, Jung Choi, Mary Ann Clark

Publisher:Matthew Douglas, Jung Choi, Mary Ann Clark

Chapter14: Dna Structure And Function

Section: Chapter Questions

Problem 13RQ: Which of the following does the enzyme primase synthesize? DNA primer RNA primer Okazaki fragments...

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Question

1. In the SDS-PAGE figure below, looking at lane 6 there are two bands MamP,
monomer and MamP dimer (disulfide bonded).
Why does the MamP monomer run faster on the gel relative to the MamP dimer?
1 2 3 4 5 6
250 -
130 -
100
70
55
- Hisg MBPtev.Mamf
MamP dimer
35 -
25-
- MamP monomer
15 -
10 -
If the gel was run under non-reducing conditions, would the pattern possibly be
different and why?
Molecular weight (kD)

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