N-acetylphenylalaninamide 2. (a) is a substrate of a-chymotrypsin. Draw the structural formula of this molecule, indicate which bond is cleaved in the hydrolytic reaction, and explain from its structure why it is a specific substrate of a-chymotrypsin. What influence does the acetyl group have on the reaction? (b) The table on the right lists initial velocity data for hydrolysis of N-acetylphenylalaninamide catal- yzed by a-chymotrypsin. From the data calculate the value of KM by a graphical method using a rectangular hyperbolic function to relate velocity to substrate con- centration and compare the results to the value obtained by a linear least-squares fit of the data in the form of a double-reciprocal plot. Substrate conc. (mm) 10.5 16.1 20.0 35.0 93.4 Vo (mmole/min) 3.33 x 10-² 4.55 x 10-² 5.56 x 10-2 7.15 x 10-² 10.0 x 10-²

N-acetylphenylalaninamide 2. (a) is a substrate of a-chymotrypsin. Draw the structural formula of this molecule, indicate which bond is cleaved in the hydrolytic reaction, and explain from its structure why it is a specific substrate of a-chymotrypsin. What influence does the acetyl group have on the reaction? (b) The table on the right lists initial velocity data for hydrolysis of N-acetylphenylalaninamide catal- yzed by a-chymotrypsin. From the data calculate the value of KM by a graphical method using a rectangular hyperbolic function to relate velocity to substrate con- centration and compare the results to the value obtained by a linear least-squares fit of the data in the form of a double-reciprocal plot. Substrate conc. (mm) 10.5 16.1 20.0 35.0 93.4 Vo (mmole/min) 3.33 x 10-² 4.55 x 10-² 5.56 x 10-2 7.15 x 10-² 10.0 x 10-²

Biochemistry

9th Edition

ISBN:9781305961135

Author:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal

Publisher:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal

Chapter6: The Behavior Of Proteins: Enzymes

Section: Chapter Questions

Problem 29RE: MATHEMATICAL The hydrolysis of a phenylalanine-containing peptide is catalyzed by -chymotrypsin with...

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