Why is it important that the alcohol used in the DNA extraction is kept cold? The solubility of any compound is reduced at lower temperatures. The colder the temperature of the alcohol, the greater the yield of DNA due to increased precipitation of DNA at the interface between the aqueous and alcohol layers. The solubility of any compound is increased at lower temperatures. The colder the temperature of the alcohol, the greater the yield of DNA due to increased solubility of DNA at the interface between the aqueous and alcohol layers. The solubility of any compound is reduced at lower temperatures. The colder the temperature of the alcohol, the greater the yield of DNA due to increased precipitation of DNA in the aqueous layer. The solubility of any compound is increased at lower temperatures. The colder the temperature of the alcohol, the greater the yield of DNA due to increased solubility of DNA in the aqueous layer.

Why is it important that the alcohol used in the DNA extraction is kept cold? The solubility of any compound is reduced at lower temperatures. The colder the temperature of the alcohol, the greater the yield of DNA due to increased precipitation of DNA at the interface between the aqueous and alcohol layers. The solubility of any compound is increased at lower temperatures. The colder the temperature of the alcohol, the greater the yield of DNA due to increased solubility of DNA at the interface between the aqueous and alcohol layers. The solubility of any compound is reduced at lower temperatures. The colder the temperature of the alcohol, the greater the yield of DNA due to increased precipitation of DNA in the aqueous layer. The solubility of any compound is increased at lower temperatures. The colder the temperature of the alcohol, the greater the yield of DNA due to increased solubility of DNA in the aqueous layer.

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter15: Genomes And Genomics

Section: Chapter Questions

Problem 6QP: Which of the following best describes the process of DNA sequencing? a. DNA is separated on a gel,...

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The last steps of DNA extraction include washing it using ethyl alcohol and drying the DNA pellet. Why do we need to wash the DNA using ice-cold ethyl alcohol? And why do we have to dry the DNA pellet?
What is the role of alcohol in extracting DNA? DNA is a polar molecule with an overall negative charge, and as such is not soluble in alcohol, and therefore precipitates. DNA is a polar molecule with an overall positive charge, and as such is not soluble in alcohol, and therefore precipitates. DNA is a non polar molecule with an overall negative change, and as such is soluble in alcohol, and therefore precipitates. DNA is a polar molecule with an overall positive change, and as such is soluble in alcohol, and therefore precipitates.
Why is it important that the alcohol used in the DNA extraction is kept cold? 1.Helps to precipitate the DNA in the alcohol layer, as the solubility of any compound is reduced at lower temperatures. The colder the temperature of alcohol, the greater the yield. 2.Helps to precipitate the DNA in the aqueous layer, as the solubility of any compound is reduced at lower temperatures. The colder the temperature of alcohol, the greater the yield. 3.Helps to precipitate the proteins at the interface between the aqueous and alcohol layers, as the solubility of any compound is reduced at lower temperatures. The colder the temperature of alcohol, the greater the yield. 4.Helps to precipitate the DNA at the interface between the aqueous and alcohol layers, as the solubility of any compound is reduced at lower temperatures. The colder the temperature of alcohol, the greater the yield.
In DNA isolation techniques, a washing step is always done prior to the final resuspension. What is the purpose of this step? In DNA isolation from blood samples, why does the vial for blood storage contain EDTA? In the preparation for DNA isolation in plants, the plant source is refrigerated and ground prior to extraction. Why is this so? Why are DNA pellets air-dried before resuspension in buffer?
Why do we need to add “COLD” alcohol? What is the effect of the temperature of the alcohol in the process of extracting the DNA.
In the protocol for extracting DNA, what is the purpose of the clear shampoo in the DNA extraction buffer? In the protocol for extracting DNA, what is the purpose of the clear shampoo in the DNA extraction buffer? Shampoo contains detergents, that help to break down lipids in cell walls, thus freeing the DNA. Shampoo contains detergents, that help to break down proteins in cell membranes, including the nuclear membrane, thus freeing the DNA. Shampoo contains salts, that help to break down lipids and proteins in cell membranes, including the nuclear membrane, thus freeing the DNA. Shampoo contains detergents, that help to break down lipids in cell membranes, including the nuclear membrane, thus freeing the DNA.
What causes the change in the ability of DNA to absorb UV light when it is denatured? options: In denatured DNA, DNA double helix is disrupted, which causes the exposure of the deoxyribose and thus increases their absorbance of UV light In denatured DNA, DNA double helix is disrupted, which causes the exposure of the phosphate groups and thus increases their absorbance of UV light. In denatured DNA, DNA double helix is disrupted, which causes the exposure of the bases and thus increases their absorbance of UV light. all of the above are correct
What is the purpose of adding table salt (NaCl) to the DNA extraction buffer? 1.Table salt dissociates into Na+ and Cl- in water The Na+ binds to negatively charged DNA and proteins, while Cl- binds to positively charged proteins, including histones, thus facilitating the separation of DNA from associated proteins, and also keeping proteins in the aqueous layer. 2.Table salt (NaCl) dissociates into Na+ and Cl- in alcohol. The Na+ binds to positively charged DNA and proteins, while Cl- binds to negatively charged proteins, including histones, thus facilitating the separation of DNA from associated proteins, and also keeping proteins in the aqueous layer. 3.Table salt (NaCl) dissociates into Na+ and Cl- in water. The Na+ binds to negatively charged DNA and proteins, while Cl- binds to positively charged proteins, including histones, thus facilitating the separation of DNA from associated proteins, and also keeping proteins in the alcohol layer. 4.Table salt dissociates…
During agarose gel electrophoresis, why does DNA move through the gel when electric current is applied? because DNA is negatively charged because a charged chemical from the loading buffer is bound to the DNA because DNA is positively charged because DNA absorbs electricity
How would the appearance of your DNA gel change if a 0.1% agarose gel slab was used? What about a 7% agarose gel slab? For separating small fragments of DNA, is it better to use a 7% gel or a 0.1% gel? Why?
What is the role of alcohol in extracting DNA? 1.DNA is a polar molecule with an overall negative charge, and as such is not soluble in alcohol, and therefore precipitates. 2.DNA is a polar molecule with an overall positive charge, and as such is not soluble in alcohol, and therefore precipitates. 3.DNA is a non polar molecule with an overall negative change, and as such is soluble in alcohol, and therefore precipitates. 4.DNA is a polar molecule with an overall positive change, and as such is soluble in alcohol, and therefore precipitates.
What are some difficulties with low copy number DNA analysis? What kinds of checks and balances can a laboratory employ to ensure reliability of DNA profiles coming from low amounts of DNA template