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- Why is heat-fixed procedure in bacterial smear preparation not ideal for capsular staining?Why is it essential that smears be air-dried? Why can’t they be gently heated over a flame to speed up the drying process?Which of the following serves as a counterstain for the gram-stain technique? It also happens to be the counterstain for the endospore staining technique.
- What are the principle and basic concepts of GRAM STAIN? (please explain it thoroughly in a lengthy but clear explanation, thank you so much.)You receive a bacterial culture in the lab and your job is to figure out information which will be helpful. You are only allowed to use one of the staining methods we practiced. Which one would be the best option? Why? And what will this information help you determine? Explain.Name all the reagents used in Gram-staining and their roles in the staining procedure.
- what effect would failing to drain the smear in-between staining have on your judgment of the gram reaction of an organism?What are the principle and basic concepts of Simple staining? (please explain it thoroughly in a lengthy but clear explanation, thank you so much.)The primary stain used in the endospore stain is: A. Carbolfuchsin Stain B. Methylene Blue C. Malachite green D. Safranine