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- As a forensic toxicologist, you have been given a task on determining amount of cadmium, mercury, chromium, copper and lead metal contents in medicinal herbs using flame atomic absorption spectroscopy (AAS). Hollow cathode lamp (HCL) was selected as radiation source for this analysis. The highest concentration came from lead element followed by chromium and copper, however cadmium and mercury were not detected in the sample. a) Based on your knowledge, conclude whether the above result is valid or not. b) If you are given an option of electrothermal or flame atomizer for above case study, determine which one is suitable and justify your answer.Draw and label a schematic of an ICP-OES spectrophotometer. Briefly (point form is acceptable) explain the purpose or function of each componentIn which technique, atomic absorption or atomic emission, is flame temperature stability more critical? Why?
- One dietary supplement capsule weighing 4.90 was ground into a fine powder. Two portions of the solid, both weighing 0.200 g, were dissolved in dilute acid and transferred to 50-mL volumetric flask. To one of these, 5.00 mL of 40.0 ppm manganese ion was added, then both flasks were diluted to the mark with distilled water. When aspirated into the flame of an atomic absorption spectrometer set at a manganese absorption wavelength, the absorbances of the unknown and unknown plus standard were 0.300 and 0.650, respectively. Calculate the amount (g) of Mn in one capsule.The measurement of Li in brine (salt water) is used by geochemists to help determine the origin of this fluid in oil fields. Flame atomic absorption of Li is subject to interference by other elements. Atomic absorption of analysis of replicate sample of Li in a brine sample gave results in the table. a. Suggest a reason for the increasing apparent concentration of Li from sample 1 to 2. b. Suggest a reason for the increasing apparent concentration of Li from sample 1 to 3 c. Why do samples 4-6 give a nearly constant result? (What type of interference you think is being eliminated?Atomic Spectroscopy (a) In terms of spectral shape, how are atomic spectra different from molecular spectra? What is the source of this difference? (b) Describe the decision-making process for picking a line for atomic emission analysis of a given analyte. (c) For quality assurance, spike recovery is not sufficient to establish confidence in an analytical procedure. Explain this statement. What should be done instead to demonstrate satisfactory performance of an instrumental method (including elemental analysis). (d) Find a literature example of atomic spectroscopy used for elemental analysis. State the operating conditions with sufficient detail to reproduce the analysis; Please answer very soon will give rating surely All questions complete Answer needed Please help me
- About Atomic spectroscopy a) Explain the fundamental difference between the two main types of atomic spectroscopy: atomic emission and atomic absorption. Also explain the main difference in instrumentation between these techniques. Draw a sketch as support. b) Explain the advantages of using emission measurements in an inductive coupled plasma (ICP) instead of flame emission measurements. Motivate! c) Measurement of mercury (for example in Värmland Lakes) can be performed with flameless atomic absorption spectroscopy. Measurement with this technique gives a detection limit of about 0.01 ppb to compare with the determination of mercury in a flame that has a significantly lower detection limit, about 200 ppb. Explain why the flameless technology is so much more sensitive for measuring mercury. Please answer very soon will give rating surely All questions answers neededA stock solution of analyte is made by dissolving 34.83 mg of copper (II) acetate hexahydrate(fw = 289.73 g/mol) in 25.00 mL of water. A second stock solution of internal standard is madeby dissolving 28.43 mg of germanium (I) acetate (fw = 190.74 g/mol) into 25.00 mL of water.These solutions are used to make a series of standards for flame atomic absorption analysiscalibration. The standard solutions (each 10.00 mL total volume) should have the followingconcentrations of copper: 10.00; 25.00; 50.00; 100.0; and 200.0 μM. Each calibration solutionshould also contain 50.00 μM of germanium. What is the analyte stock solution concentration? What is the internal standard stocksolution concentration?Complete this table.An analyst notes that a 1 ppm solution of sodium gives a flame emission signal of 110. While the same solution containing also 20 ppm potassium gives a reading of 125. It was determined that a 20 ppm solution of potassium exhibited no blank reading. Explain the results?
- using the following information to construct a valid hypothesis addressing the effect of pH and temperature on the hydrolysis of starch. Per bench, prepare the stock amylase enzyme solution by adding distilled water to the powdered enzyme in the flask , each bench will share one flaks of enzyme solution. per groups, turn on the spectrometer and set the wavelength to 560nm.allow the instruments to warm up for at least 10 minutes. per group, set up 17 cuvettes in a Styrofoam rack. Add 3 drops of iodine (IKI) to each coveter. per group, prepare the “black” cuvette by rapidly adding 3 mL of maltose standard solution to one of The prepared cuvettes containing IKI. Rapid addition of the maltose will mix the maltose and IKIOne dietary supplement capsule weighing 4.90 was ground into a fine powder. Two portions of the solid, both weighing 0.200 g, were dissolved in dilute acid, and transferred to 50-mL volumetric flask. To one of these, 5.00 mL of 40.0 ppm manganese ion was added, then both flasks were diluted to the mark with distilled water. When aspirated into the flame of an atomic absorption spectrometer set at a manganese absorption wavelength, the absorbances of the unknown and unknown plus standard were 0.300 and 0.650, respectively. What is the type of calibration method used? a) Standard Addition b) Internal Standard c) External StandardA 0.0140 M solution of a certain analyte exhibits 49.8% T' at a wavelength of 280 nm. What will the percent transmittance be for a 0.0252 M solution of the same analyte? Assume the pathlengths for each measurement are equal.
