Q: name the six different types of vectors?
A: Vector is DNA molecule that is used as a vehicle to artificially carry the gene of interest using…
Q: Stable transmission of linear yeast artificial chromosomes(YACs) requires?
A: Yeast artificial chromosomes (YACs) are genetically engineered chromosomes derived from the DNA of…
Q: What three essential criteria must be met in order to execute asuccessful mapping cross?
A: Introduction: A genetic cross is the mating of two individuals with desired characters. The…
Q: Which vector is used in the manufacturing of Covishield?
A: The delivery of vaccines require the presence of vector so that the virus can be transferred to the…
Q: What is the role of PopZ in segregating theCaulobacter daughter chromosomes?
A: Caulobacter crescentus is a gram-negative alpha-proteobacterium. It contains a single circular…
Q: What are the steps in making an mRNA vaccine?
A: mRNA vaccines combine ideal immunological houses with an exceptional protection profile and the…
Q: In what major way does transcription of phage DNA differ inphages T4 and T7?
A: The central dogma of the protein synthesis process involves transcription and translation.…
Q: Mention any three vector-less methods that are used to introduce recombinant DNA into a competent…
A: Micropropagation ,electroporation and gene gun methods are three vectorless methods that are used to…
Q: Describe the role that integrase plays during the insertion of λDNA into the host chromosome.
A: Integrase is a viral enzyme that catalyzes the integration of virally derived DNA into host cell…
Q: Discuss the lysogrnic induction of bacteriophage lambda in coli cells
A: Viruses are microbes that have both living and non-living characteristics. They are obligate…
Q: Why are vent polymerase and Pfu more efficient than the Taq polymerase?
A: Polymerase is an enzyme that forms nucleic acids’ long chains. The RNA and DNA polymerase are used…
Q: What does the following vector produce? Be specific.
A: Vector is defined as a DNA molecule that is used as a vehicle to carry foreign genetic material into…
Q: Describe the method of pronuclear injection to generate transgenic mice?
A: Transgenic mice are produced by putting DNA in their genome from another source. There are a number…
Q: What is the causitive agent of covid 19?
A: Causative agents are those biological pathogens that cause a disease, such as a virus, parasite,…
Q: Would the application of paclitaxel to a cell make us incapable of performing karyotyping on that…
A: Karyotyoing is the process by which DNA is analyzed . The chromosome structure is usually studied .
Q: Discuss how to transform the yeast vector, YEP containing your gene of interest into the host cell.
A: To insert a set of desirable foreign genes into the host genome for replication and expression, a…
Q: Describe how the lambda phage controls the lytic and lysogenic cycles. Include cro, cI, cII, and…
A: The mature Lambda virus particle consists of a linear double helical DNA molecule surrounded by a…
Q: For what purpose are m13-derived vectors especially useful? What feature (s) of the m13 life cycle…
A: M13 bacteriophage is a filamentous bacteriophage that composed of 6407 nucleotides long circular…
Q: What is cotransduction? What determines the likelihood that two genes will be cotransduced?
A: We have to have the knowledge of genetics to answer this question.
Q: Discuss the key events in the production of recombinant subunit vaccine?
A: The technology used for joining different DNA molecules from different species is known as…
Q: What is the difference between a l insertion vector such as lgt11 and a l replacement vector?
A: Vector in molecular biology is a DNA molecule which carries foreign genetic material into foreign…
Q: Draw the map of the Hfr chromosome and indicate the position of the F factor, the direction of the…
A: Answer: Hfr cell : These are the High Frequency Recombination bacterial cells with plasmid…
Q: What transcritpion factor is produced in the Nieuwkoop center in reponse to nuclear beta-catenin?(…
A: Transcription factors are usually the protein that have the capacity to increase or decrease the…
Q: True or False: Hybridoma cells are used to produceantigens designed to attack one and only one site…
A: The monoclonal antibodies are used in the forensic test kits. Most of the immunoassay test kits for…
Q: What mechanism in the mRNA covid-19 vaccine allows the immune system to recognize the cell as…
A: mRNA vaccines are new type of vaccine to protect against infectious diseases.In order to trigger the…
Q: State the differences between the plasmid in Figure 1 with pBR322 in terms of their sercening…
A: According to the question, we have to state the differences between the plasmid in figure 1 with…
Q: how does 1. Low extracellular concentrations of inorganic iron, and 2. lysogenic prophage that…
A: Two components have extraordinary impact on the capacity of Corynebacterium diphtheriae to create…
Q: Explain, the mechanism of chaperone mediated autophagy.
A: Molecular chaperones are proteins that help with conformational folding and unfolding, as well as…
Q: What part of the A. tumefaciens DNA gets transferred to the genome of a plant cell during infection?
A: The genome represents the genetic material in an organism having a set of chromosomes which is…
Q: Which of the following are true for the c-myc gene? Select all that apply
A: Myc is a group of regulator genes that code for transcription factors. c-myc is one of the genes in…
Q: Please explain 5’capping and its for the cell?
A: 5’ refers to the 5 prime capping which means 5 prime cap is a unique altered nucleotide on the 5’…
Q: Describe how you would genetically design a superbugresistant to b-lactams, methicillin,…
A: Superbugs are strains of viruses, bacteria, fungi, and parasites that are resistant to most the…
Q: What is the etiology of COVID 19? and its variants? and How is it being spread and how long is its…
A: Introduction Coronavirus disease 2019 (COVID-19) is a contagious disease caused by the coronavirus…
Q: Describe any three vectors less method of introducing the rDNA into a competent host cell?
A: rDNA is the recombinant DNA which is formed by combining desired genes from different organisms. It…
Q: Describe sectored colonies in yeast and theirsignificance in evaluating mitotic recombination.
A: Mitosis is the process where chromosomes divide to produce two identical nuclei before being packed…
Q: Describe how ES cells are used to generate knockoutmice.
A: Knockout mice is a genetically modified mice in which the researchers have inactivated or knocked…
Q: Why is generalized transduction able to transfer anygene, but specialized transduction is restricted…
A: Introduction: transduction is a process of gene transfer in bacteria from donor to recipient…
Q: How can the yeast vector, YEP, containing your desired gene, be inserted into the host cell? Discuss…
A: YEp stands for Yeast Episomal plasmid is a shuttle vector. Shuttle vectors are vectors designed in a…
Q: Explain what is the agglutination reaction: WHAT agglutinates with what? Agglutination is the…
A: Agglutination reaction is related with the blood group of humans. Blood type in humans is broadly…
Q: What are the roles of Cas1, Cas2, and Cas9 proteins in bacterialgenome defense?
A: Phages are the bacteria killers that inject their DNA into the bacteria and uses the bacterial…
Q: In ELISPOT Assay, explain the importance of adding stimulus to the 96-well plate during the…
A: ELISPOT assay is an Enzyme-Linked Immune adsorbent Spot assay that is used for testing cellular…
Q: Describe the three different methods of horizontal gene transfer among bacteria and mention their…
A: Horizontal gene transfer, otherwise called lateral gene transfer, is an interaction where an…
Q: How can we analyze the interest protein expression in cultured cells after we transfect with the…
A: Protein tagged with green fluorescent protein can be analyzed by using fluorescence microscopy since…
Q: We are utilizing BL21 DE3 bacterial cells for the expression of the ADA protein via autoinduction.…
A: BL21 DE3 Bacterial cells: It is an E. coli B strain that lacks the Lon protease. Recombinant…
Q: What must a vector contain?
A: Vector is a nucleic acid molecule containing Viral DNA or RNA and plasmid yeast or bacterial…
Q: What is the visibility of proteomic phenotype in COVID-19 patients?
A: The protein is defined because the set of proteins that an organism will manufacture. By…
Name the commonly used vector for trans formation in plant cell?
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- Draw and label the E. coli host cell/T4 bacteriophage interaction.?Approximately how many genes are necessary for amicrobial cell to have a free-living existence?how does 1. Low extracellular concentrations of inorganic iron, and 2. lysogenic prophage that contains the tox gene present in becterium chromosome. control the transcription and expression of the Diphtheria toxin for C. diphtheriae. Explain separately.