Please help! Im confused 7.7.NATIONAL CENTER FOR CASE STUDY TEACHING IN SCIENCENATIONAL CENTER FOR CASE STUDY TEACHING IN SCIENCEApplying High-ThroughputAnalysis to BiofilmsQuestions1. Using the information provided, complete the Treatment column in the following table and consider what theprocedures have in common.Table 1: Treatment received by patient and presence of bacterial infection.буMichael L. Homesley, Jr.Department of PhysiologyNorth Carolina State University, Raleigh, NCPatientTreatment+/- for Mycobacterium abscessusMark+StephenCarlaPart I- Dentistry MysteryScottDr. S was a general dentist practicing in the western mountains of North Carolina, welcoming patients with a widevariety of needs into his office. The week of March 13th had been much like any other at DDS Family Dentistry, withthe seats of the lobby filled daily with people anxiously waiting to receive care. However, the following week the officereceived a call that all practitioners dread-the health inspector was en route to assess the condition of the workplace.What was the reason for the unexpected inspection?Albert+Samuel+OliviaJaylenLet's backtrack to review some of the care that was administered during the week in question in order to establish thecause for the inspector's visit. The following patients were seen on Thursday, March 16th :Mark had been a smokeless tobacco user for nearly 25 years. His lower right molars had become so severelydecayed that he was in excruciating pain. Dr. S offered the option to extract the teeth, and Mark decided tohave the procedure performed immediately.Doris+Brian+2. According to Table 1, what do you think could be the source of contamination leading to the infection? Use thefollowing information about the various procedures the patients underwent to help you.Fourteen-year old Stephen had a biking accident. His front tooth hit the handlebars and knocked the bracketloose and bent the wire in his braces. Dr. S determined that no additional damage was done besides the oneloose bracket and was able to complete the repair of the braces.Carla, Scott, Albert, and Samuel all came in for their bi-annual checkup. An oral examination revealed nodisease, so all of these patients received routine teeth cleaning.Olivia had prom in two months and was not happy with her smile. She came in a week prior for impressions tobe taken so that trays could be fabricated to deliver whitening product. Her trays were delivered on Monday, soshe had come in to pick them up and receive instruction for use.Teeth extraction: teeth extracted using sanitized instruments, area rinsed with water from dental unit waterline(DUWL), and gauze secured.Repair of broken braces bracket: tooth lightly air-dried, bracket bonded into place, adhesive cured.Bi-annual checkup: scaling, polishing, flossing, and rinsing of teeth from DUWL, along with complete oralexamination with sterilized tools.Whitening tray pickup: patient received whitening trays and supplied with whitening gel.Jaylen had been dealing with what he thought was an ulcer, but after using multiple over-the-counter (OTC)rinses for several weeks and seeing no improvement, he decided to make an appointment with Dr. S. After anoral examination, it was determined that a segment of metal had frayed on his bridge appliance. Thewas clipped off using a pair of sterilized pliers, and Jaylen's pain was alleviated.Bridge repair: anchor adjusted using sterilized instruments.Denture checkup: appliance removed and adjusted, rinsed with water from DUWL, and re-inserted for patient.Fitting for retainer: impressions taken of teeth and mouth rinsed with water from DUWL.strayendDoris was scheduled for her annual checkup for her dentures. Dr. S observed signs of a malocclusion (in thiscase, crossbite) and made a minor adjustment by removing a small portion of several denture teeth using thehigh-speed handpiece. After rinsing off the appliance using water from the dental unit, the denture was placedback in the patient's mouth. A few bites on the articulating paper to check the contacts between the teeth madeit clear that the problem had been solved.3. Based on your answer above, is this source an ideal environment for the formation of microbial biofilms?Brian had his braces removed last month and had already managed to lose his retainers. He came into the officein order to have new retainers made.It was now a week later, and further discussion with the health inspector revealed that multiple patients had beenadmitted to the hospital with the same Mycobacterium abscessus bacterial infection. The factor they shared in commonwas a recent visit to DDS Family Dentistry. A thorough investigation of the facility was deemed necessary and patientstreated within the same timeframe were notified about the apparent contamination and encouraged to visit theirprimary physician in order to be tested for the presence of this bacterial organism. Mycobacterium abscessus has beenlinked to the development of a "wide spectrum of skin and soft tissue diseases" (Lee et al., 2015)."Applying High-Throughput Analysis to Biofilms" by Michael L. Homesley, Jr.Page 1“Applying High-Throughput Analysis to Biofilms" by Michael L. Homesley, Jr.Page 22 of 6

Question

Please help! Im confused 7.7.NATIONAL CENTER FOR CASE STUDY TEACHING IN SCIENCENATIONAL CENTER FOR CASE STUDY TEACHING IN SCIENCEApplying High-ThroughputAnalysis to BiofilmsQuestions1. Using the information provided, complete the Treatment column in the following table and consider what theprocedures have in common.Table 1: Treatment received by patient and presence of bacterial infection.буMichael L. Homesley, Jr.Department of PhysiologyNorth Carolina State University, Raleigh, NCPatientTreatment+/- for Mycobacterium abscessusMark+StephenCarlaPart I- Dentistry MysteryScottDr. S was a general dentist practicing in the western mountains of North Carolina, welcoming patients with a widevariety of needs into his office. The week of March 13th had been much like any other at DDS Family Dentistry, withthe seats of the lobby filled daily with people anxiously waiting to receive care. However, the following week the officereceived a call that all practitioners dread-the health inspector was en route to assess the condition of the workplace.What was the reason for the unexpected inspection?Albert+Samuel+OliviaJaylenLet's backtrack to review some of the care that was administered during the week in question in order to establish thecause for the inspector's visit. The following patients were seen on Thursday, March 16th :Mark had been a smokeless tobacco user for nearly 25 years. His lower right molars had become so severelydecayed that he was in excruciating pain. Dr. S offered the option to extract the teeth, and Mark decided tohave the procedure performed immediately.Doris+Brian+2. According to Table 1, what do you think could be the source of contamination leading to the infection? Use thefollowing information about the various procedures the patients underwent to help you.Fourteen-year old Stephen had a biking accident. His front tooth hit the handlebars and knocked the bracketloose and bent the wire in his braces. Dr. S determined that no additional damage was done besides the oneloose bracket and was able to complete the repair of the braces.Carla, Scott, Albert, and Samuel all came in for their bi-annual checkup. An oral examination revealed nodisease, so all of these patients received routine teeth cleaning.Olivia had prom in two months and was not happy with her smile. She came in a week prior for impressions tobe taken so that trays could be fabricated to deliver whitening product. Her trays were delivered on Monday, soshe had come in to pick them up and receive instruction for use.Teeth extraction: teeth extracted using sanitized instruments, area rinsed with water from dental unit waterline(DUWL), and gauze secured.Repair of broken braces bracket: tooth lightly air-dried, bracket bonded into place, adhesive cured.Bi-annual checkup: scaling, polishing, flossing, and rinsing of teeth from DUWL, along with complete oralexamination with sterilized tools.Whitening tray pickup: patient received whitening trays and supplied with whitening gel.Jaylen had been dealing with what he thought was an ulcer, but after using multiple over-the-counter (OTC)rinses for several weeks and seeing no improvement, he decided to make an appointment with Dr. S. After anoral examination, it was determined that a segment of metal had frayed on his bridge appliance. Thewas clipped off using a pair of sterilized pliers, and Jaylen's pain was alleviated.Bridge repair: anchor adjusted using sterilized instruments.Denture checkup: appliance removed and adjusted, rinsed with water from DUWL, and re-inserted for patient.Fitting for retainer: impressions taken of teeth and mouth rinsed with water from DUWL.strayendDoris was scheduled for her annual checkup for her dentures. Dr. S observed signs of a malocclusion (in thiscase, crossbite) and made a minor adjustment by removing a small portion of several denture teeth using thehigh-speed handpiece. After rinsing off the appliance using water from the dental unit, the denture was placedback in the patient's mouth. A few bites on the articulating paper to check the contacts between the teeth madeit clear that the problem had been solved.3. Based on your answer above, is this source an ideal environment for the formation of microbial biofilms?Brian had his braces removed last month and had already managed to lose his retainers. He came into the officein order to have new retainers made.It was now a week later, and further discussion with the health inspector revealed that multiple patients had beenadmitted to the hospital with the same Mycobacterium abscessus bacterial infection. The factor they shared in commonwas a recent visit to DDS Family Dentistry. A thorough investigation of the facility was deemed necessary and patientstreated within the same timeframe were notified about the apparent contamination and encouraged to visit theirprimary physician in order to be tested for the presence of this bacterial organism. Mycobacterium abscessus has beenlinked to the development of a &#34;wide spectrum of skin and soft tissue diseases&#34; (Lee et al., 2015).&#34;Applying High-Throughput Analysis to Biofilms&#34; by Michael L. Homesley, Jr.Page 1“Applying High-Throughput Analysis to Biofilms&#34; by Michael L. Homesley, Jr.Page 22 of 6

Accepted Answer

1. Mark had his lower molar tooth extraction done . The steps are:

1. Numbing the tooth with…