Nocodazole is a potent inhibitor of microtubule polymerization, which is required for formation of the mitotic spindle. By treating a population of mammalian cells with nocodazole for a time and then washing it out of the medium, we can synchronize the cell population. In the presence of nocodazole, where in the cell cycle do you expect the cells to accumulate? What mechanism do think is responsible for stopping cell-cycle progression when cells are treated with nocodazole? you

Nocodazole is a potent inhibitor of microtubule polymerization, which is required for formation of the mitotic spindle. By treating a population of mammalian cells with nocodazole for a time and then washing it out of the medium, we can synchronize the cell population. In the presence of nocodazole, where in the cell cycle do you expect the cells to accumulate? What mechanism do think is responsible for stopping cell-cycle progression when cells are treated with nocodazole? you

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter10: Cell Division And Mitosis

Section: Chapter Questions

Problem 8TYK: Which of the following statements about cell cycle regulators is incorrect? a. The concentrations of...

See similar textbooks

Similar questions

Biologists have long been interested in the effects of radiation on cells. In one experiment, researchers examined the effect of radium on mitosis of chick embryo cells growing in culture. A population of experimental cells was examined under the microscope for the number of cells in telophase (as a measure of mitosis occurring) before, during, and after exposure to radium. The results are shown in the Figure. What is the effect of radium exposure on mitosis? Source: R. G. Canti and M. Donaldson. 1926. The effect of radium on mitosis in vitro. Proceedings of the Royal Society of London, Series B, Containing Papers of a Biological Character 100:413419.
Which statements are true? Explain why or why not.1 Since there are about 1013 cells in an adult human,and about 1010 cells die and are replaced each day, webecome new people every three years.2 In order for proliferating cells to maintain a rela-tively constant size, the length of the cell cycle must matchthe time it takes for the cell to double in size.3 While other proteins come and go during thecell cycle, the proteins of the origin recognition complexremain bound to the DNA throughout.4 Chromosomes are positioned on the metaphaseplate by equal and opposite forces that pull them towardthe two poles of the spindle.5 Meiosis segregates the paternal homologs intosperm and the maternal homologs into eggs.6 If we could turn on telomerase activity in all ourcells, we could prevent aging.
The cell cycle of an average mammalian cell is illustrated below. How long did it take for the above-illustrated mammalian cell to complete one cell cycle?How long did the mitosis/cytokinesis phase (M phase) last? What % of the total cell-cycle timedoes that constitute to? What phase takes the longest time to complete during cell cycle and what % of the total cellcycle time does that constitute to? Explain why you think this might be the longest phase of thecell cycle.How long was the S phase? What is happening in the cell during the S phase?
When studying living cells in a laboratory, researchers sometimesuse drugs as a way to cause cells to remain in a particular phaseof the cell cycle. For example, aphidicolin inhibits DNA synthesisin eukaryotic cells and causes them to remain in the G1 phasebecause they cannot replicate their DNA. In what phase of thecell cycle—G1, S, G2, prophase, metaphase, anaphase, or telophase—would you expect somatic cells to stay if the followingtypes of drug were added?A. A drug that inhibits microtubule formationB. A drug that allows microtubules to form but prevents themfrom shorteningC. A drug that inhibits cytokinesisD. A drug that prevents chromosomal condensation
Passage of cells from G1 through R to S depends on the interaction of various signal molecules, proteins and enzymes which regulate the cell cycle. Imagine I have just induced a mutation in a cell line which prevents the breakdown of the cyclin molecule we discussed. Which of the following events do you predict will happen? circle all that apply Select one or more: a. Uncontrolled cell replication may result b. Retinoblastoma protein will be continuously active c. The cells will be stuck in G1 phase d. The cells will begin to replicate their DNA e. Cyclin dependent kinase (Cdk) will be continuously active
f you are growing mammalian cells in culture that are in G0, which of the following would you add to the cell culture media to stimulate them to leave G0 and enter the cell cycle? A. growth factors or other growth stimulatory molecules B. Taq polymerase C. Tau D. BrdU E. Na+ ions
One approach to studying the regulation of cell cycle progression (particularly in an era when genetic and molecular biology manipulations were less readily accomplished in mammalian cells) was to use treatments that induced cells to fuse and then monitor the behavior of the two nuclei in the resulting cell. The figure below depicts data from one such study. The investigators did preliminary work to produce populations of cells that were synchronized in various stages of the cell cycle (G1, S, or G2 in the examples shown below). They then fused the cells in different combinations and monitored subsequent events in each of the nuclei. For purposes of this question, we will pay particular attention to what occurred in the nucleus that came from the cell in G1. In one experiment (I), cells in the G1 and S phases were fused. That event caused the nucleus from the G1 cell to very quickly enter the S phase (sooner than it would otherwise have done so). In contrast, in a second experiment…
One approach to studying the regulation of cell cycle progression (particularly in an era when genetic and molecular biology manipulations were less readily accomplished in mammalian cells) was to use treatments that induced cells to fuse and then monitor the behavior of the two nuclei in the resulting cell. The figure below depicts data from one such study. The investigators did preliminary work to produce populations of cells that were synchronized in various stages of the cell cycle (G1, S, or G2 in the examples shown below). They then fused the cells in different combinations and monitored subsequent events in each of the nuclei. For purposes of this question, we will pay particular attention to what occurred in the nucleus that came from the cell in G1. In one experiment (I), cells in the G1 and S phases were fused. That event caused the nucleus from the G1 cell to very quickly enter the S phase (sooner than it would otherwise have done so). In contrast, in a second experiment…
When studying living cells in a laboratory, researchers sometimesuse drugs as a way to make cells remain at a particular stage of thecell cycle. For example, aphidicolin is an antibiotic that inhibitsDNA synthesis in eukaryotic cells and causes them to remain inthe G1 phase because they cannot replicate their DNA. In whatphase of the cell cycle—G1, S, G2, prophase, metaphase, anaphase,or telophase—would you expect somatic cells to stay if they weretreated with each of the following types of drug?A. A drug that inhibits microtubule formationB. A drug that allows microtubules to form but prevents them fromshorteningC. A drug that inhibits cytokinesisD. A drug that prevents chromosomal condensation
Kinetochores control the transition from metaphase to anaphase. Why is this statement true? Question 7 options: a) Anaphase will only begin after the M checkpoint where all the kinetochores and mitotic spindle have correctly attached. b) Anaphase will only begin after the M checkpoint where all the mitotic spindle have correctly formed. c) Kinetochores bind to microtubules in monotelic attachment to the sister chromatids. d) Kinetochores bind to microtubules in syntelic attachment to the sister chromatids. e) Kinetochores will halt at the M checkpoint to weaken the mitotic spindle formation, which is the event that initiates anaphase.
Choose all of the TRUE statements. Hint: 4 statements are true. Cytokinesis occurs the same way in both plant and animal cells. Sister chromatids are genetically identical. The term chromosome can be used to describe both chromosomes composed of one and two chromatids. Mitosis results in daughter cells that are genetically identical to the parent cell. All eukaryotic somatic cells are diploid. Cyclin dependent kinases are present but not active in cell cycle regulation without the presence of cyclin proteins. Chromosomes are always visible in the cell.
If I extract a cell culture with 100 cells that just entered g1. I needs to extract the MOST possible DNA from 100 cells. On top of that, it requires that the DNA cannot be condensed. To accomplish this goal he gives you a set of chemicals that will stop a cell in any part of the cell cycle you want. What part of the cell cycle would you stop the cell at, that matches those requirements? PermalinkReply